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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Inhibition of glutathione S-transferase omega 1-catalyzed protein deglutathionylation suppresses adipocyte differentiation

  • Sana Iram (Department of Medical Biotechnology, Yeungnam University) ;
  • Areeba Mashaal (Department of Medical Biotechnology, Yeungnam University) ;
  • Seulgi Go (Department of Medical Biotechnology, Yeungnam University) ;
  • Jihoe Kim (Department of Medical Biotechnology, Yeungnam University)
  • Received : 2023.03.21
  • Accepted : 2023.04.25
  • Published : 2023.08.31
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Abstract

Glutathione S-transferase omega 1 (GstO1) is closely associated with various human diseases, including obesity and diabetes, but its functional mechanism is not fully understood. In the present study, we found that the GstO1-specific inhibitor C1-27 effectively suppressed the adipocyte differentiation of 3T3-L1 preadipocytes. GstO1 expression was immediately upregulated upon the induction of adipocyte differentiation, and barely altered by C1-27. However, C1-27 significantly decreased the stability of GstO1. Moreover, GstO1 catalyzed the deglutathionylation of cellular proteins during the early phase of adipocyte differentiation, and C1-27 inhibited this activity. These results demonstrate that GstO1 is involved in adipocyte differentiation by catalyzing the deglutathionylation of proteins critical for the early phase of adipocyte differentiation.

Keywords

Acknowledgement

This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2020R1A6A1A03044512 and 2020R1I1A3060716).

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