Mycobiology

The Korean Society of Mycology (한국균학회)
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Molecular Phylogeny and Morphology of Tolypocladium globosum sp. nov. Isolated from Soil in Korea

  • Kallol Das (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Jung-Joo Ryu (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Soo-Min Hong (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Seong-Keun Lim (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Seung-Yeol Lee (College of Agriculture and Life Sciences, Kyungpook National University) ;
  • Hee-Young Jung (College of Agriculture and Life Sciences, Kyungpook National University)
  • Received : 2023.02.03
  • Accepted : 2023.03.15
  • Published : 2023.04.30
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Abstract

In this study, fungal strains designated as KNUF-22-14A and KNUF-22-15A were isolated from soil samples in Korea. These two strains were identified based on cultural and morphological characteristics as well as phylogenetic analyses and were found to be morphologically and phylogenetically identical. Upon their morphological comparison with closely related species, such as Tolypocladium album, T. amazonense, T. endophyticum, T. pustulatum, and T. tropicale, a difference in the size of short phialides [0.6-2.4(-9.3)×0.8-1.4 ㎛] was observed. Meanwhile, these strains had larger conidia (1.2-3.0×1.2-3.0 ㎛) than T. album, T. amazonense, T. endophyticum, and T. tropicale and smaller conidia than T. pustulatum. Phylogenetic analyses using a multi-locus datasets based on ITS, LSU, and SSU showed that KNUF-22-14A and KNUF-22-15A formed a distinct cluster from previously identified Tolypocladium species. Thus, these fungal strains isolated from soil in Korea are proposed as a novel species according to their characteristics and are named Tolypocladium globosum sp. nov.

Keywords

Acknowledgement

This work was supported by a grant from the National Institute of Biological Resources, funded by the Ministry of Environment of the Republic of Korea (NIBR202304104).

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