Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Immunostimulatory Activity of Syneilesis palmata Leaves through Macrophage Activation and Macrophage Autophagy in Mouse Macrophages, RAW264.7 Cells

  • So Jung Park (Department of Forest Science, Andong National University) ;
  • Jeong Won Choi (Department of Forest Science, Andong National University) ;
  • Hyeok Jin Choi (Department of Forest Science, Andong National University) ;
  • Seung Woo Im (Department of Forest Science, Andong National University) ;
  • Jin Boo Jeong (Department of Forest Science, Andong National University)
  • Received : 2023.01.30
  • Accepted : 2023.04.12
  • Published : 2023.07.28
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Abstract

Syneilesis palmata (SP) is a traditional medicinal plant. SP has been reported to have anti-inflammatory, anticancer, and anti-human immunodeficiency virus (HIV) activities. However, there is currently no research available on the immunostimulatory activity of SP. Therefore, in this study, we report that S. palmata leaves (SPL) activate macrophages. Increased secretion of both immunostimulatory mediators and phagocytic activity was observed in SPL-treated RAW264.7 cells. However, this effect was reversed by the inhibition of TLR2/4. In addition, inhibition of p38 decreased the secretion of immunostimulatory mediators induced by SPL, and inhibition of TLR2/4 decreased the phosphorylation of p38 induced by SPL. SPL augmented p62/SQSTM1 and LC3-II expression. The increase in protein levels of p62/SQSTM1 and LC3-II induced by SPL was decreased by the inhibition of TLR2/4. The results obtained from this study suggest that SPL activates macrophages via TLR2/4-dependent p38 activation and induces autophagy in macrophages via TLR2/4 stimulation.

Keywords

Acknowledgement

This work was supported by the R&D Program for Forest Science Technology (Project No. 2021377C10-2123-BD02) provided by the Korea Forest Service (Korea Forestry Promotion Institute) and the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF2018R1A6A1A03024862).

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