Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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First report of red bean brown leaf blight caused by Rhizopus arrhizus (syn. R. oryzae)

Rhizopus arrhizus (syn. R. oryzae)에 의한 팥 갈색잎마름병 첫 보고

  • Seongkyun Lee (Chungbuk Agricultural Research and Extension Services) ;
  • Jieun Yeon (Chungbuk Agricultural Research and Extension Services) ;
  • Eunsol Yeon (Chungbuk Agricultural Research and Extension Services) ;
  • Jongok Jeon (Chungbuk Agricultural Research and Extension Services) ;
  • Hyunman Shin (Chungbuk Agricultural Research and Extension Services) ;
  • Ju-Hyung Kim (Chungbuk Agricultural Research and Extension Services) ;
  • Sangbeom Lee (Chungbuk Agricultural Research and Extension Services)
  • 이성균 (충청북도농업기술원) ;
  • 연지은 (충청북도농업기술원) ;
  • 연은솔 (충청북도농업기술원) ;
  • 전종옥 (충청북도농업기술원) ;
  • 신현만 (충청북도농업기술원) ;
  • 김주형 (충청북도농업기술원) ;
  • 이상범 (충청북도농업기술원)
  • Received : 2023.11.10
  • Accepted : 2023.12.26
  • Published : 2023.12.31
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Abstract

Milky white spots appeared on red bean leaves in a red bean cultivation area located in Jangyeon-myeon, Goesan-gun, Chungbuk Province. After culturing the pathogen in PDA medium, their morphology was observed, and their genes were BLAST-searched in the National Center for Biotechnology Information (NCBI). The pathogen was identified as a fungus called Rhizopus arrhizus. As a result of reinoculating the isolated pathogen on red beans, the same symptoms as those in the isolated leaves occurred. Characteristic colonies of R. arrhizuson PDA medium initially showed a bright color and then changed to dark gray over time, with mostly spherical sporangia. The sporangiospores were spherical or elliptical, mostly irregular, and small in size. Therefore, based on these results, this disease has not yet been reported in red beans and was called red bean brown leaf blight caused by Rhizopus arrhizus A. Fisch (syn. R. oryzae).

충북 괴산군 장연면에 위치한 팥 재배지에서 팥잎에 유백색 반점이 생기고 점차 시간이 지남에 따라 감염 부위가 갈색으로 변하는 증상을 발견하였다. PDA 배지에서 병원균을 배양 후 형태를 관찰하고 유전자를 NCBI에서 BLAST search한 결과 R. arrhizus라는 진균으로 동정되었다. 또한 분리한 병원균을 팥에 다시 접종한 결과 분리한 잎에서 나타난 증상과 동일한 증상이 발생하여 팥에 병원성이 있는 것으로 확인되었다. R. arrhizus의 특징으로 PDA 배지에서 균총은 초기에 밝은색을 띠다가 시간이 지남에 따라 어두운 회색으로 변하였으며, 포자낭은 대부분 구형이었다. 포자낭포자의 경우 구형 또는 타원형으로 대부분이 불규칙하고 크기가 작았다. 따라서 이와 같은 결과를 토대로 이 병은 지금까지 팥에 보고되지 않은 병으로서 Rhizopus arrhizus A. Fisch (syn. R. oryzae)에 의한 팥 갈색잎마름병으로 명명하고자 한다.

Keywords

Acknowledgement

This research was supported by a grant from the Agenda Program (RS-2021-RD009863), funded by the Rural Development Administration of Korea.

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