Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Assessment of the toxic effects of dichlofluanid using survival and relative growth rate on brown alga Undaria pinnatifida

미역(Undaria pinnatifida) 배우체의 생존 및 상대성장률을 이용한 dichlofluanid의 독성영향 평가

  • Un-Ki Hwang (Tidal Flat Research Center, NIFS) ;
  • Yun-Ho Park (Risk Assessment Division, Environmental Health Research Department, NIER) ;
  • Bo-Ram Sim (West Sea Fisheries Research Institute, NIFS) ;
  • Ju-Wook Lee (West Sea Fisheries Research Institute, NIFS)
  • 황운기 (국립수산과학원 기후환경연구부 갯벌연구센터) ;
  • 박윤호 (국립환경과학원 환경건강연구부 위해성평가연구과) ;
  • 심보람 (국립수산과학원 서해수산연구소 기후환경자원과) ;
  • 이주욱 (국립수산과학원 서해수산연구소 기후환경자원과)
  • Received : 2023.09.19
  • Accepted : 2023.11.08
  • Published : 2023.12.31
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Abstract

Biocide dichlofluanid breaks down quickly and accumulates easily in sediment, potentially causing a persistent impact on various marine organisms. We analyzed the potential toxicity of dichlofluanid on major aquaculture species in Korea, Undaria pinnatifida. Female gametophytes of U. pinnatifida were exposed to dichlofluanid at concentrations of 0, 1, 2, 4, 8, 16, and 32 mg L-1, and their survival and relative growth rate were analyzed. The no observed effect concentration(NOEC), lowest observed effect concentration (LOEC), and median lethal concentration (LC50) for female gametophyte survival were determined as 1, 2, and 10.82 (95% CI: 8.87-13.23) mg L-1, respectively. The NOEC, LOEC, and median effective concentration (EC50) for relative growth rate were 1, 2, and 6.58 (95% CI: 6.03-7.17) mg L-1, respectively. Female gametophytes of U. pinnatifida were expected to experience toxic effects at concentrations above 2 mg L-1 of dichlofluanid. These research findings are expected to serve as important reference data for evaluating the toxicity effects of U. pinnatifida in its early life stages when exposed to dichlofluanid.

Dichlofluanid는 해양환경 내 빠르게 분해되지만 농약 및 방오제로 사용되고 퇴적물에 쉽게 축적되어 지속적으로 해양환경으로 유입되고 있다. Dichlofluanid는 다양한 해양생물에게 독성영향을 미치는 것으로 알려져 있지만 해조류에 대한 독성연구는 충분히 보고되지 않았다. 따라서 우리나라의 주요 양식생물인 미역(Undaria pinnatifida) 암배우체의 생존율 및 상대성장률을 이용하여 dichlofluanid의 독성영향을 분석하였다. U. pinnatifida의 암배우체를 dichlofluanid (0, 1, 2, 4, 8, 16, 32 mg L-1)에 노출하였고 암배우체 생존율의 무영향농도(NOEC), 최소영향농도(LOEC), 반수치사농도(LC50)는 1, 2, 10.82(8.87~13.23) mg L-1, 상대성장률의 NOEC, LOEC, 반수영향농도(EC50)은 1, 2, 6.58 (6.03~7.17) mg L-1로 분석되었다. 본 연구결과는 dichlofluanid에 대한 U. pinnatifida 암배우체의 독성영향을 평가하기 위한 중요한 참고자료로 활용될 것으로 기대된다.

Keywords

Acknowledgement

본 연구는 2023년 국립수산과학원 갯벌연구센터(R2023027)의 연구비 지원으로 수행되었다.

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