Journal of Radiation Industry (방사선산업학회지)

Korean Society of Radiation Industry (한국방사선산업학회)
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Evaluation on the Applicability of Comet Assay for the Identification of Gamma-irradiated Frozen Beef Cut, Ground Beef and Tteokgalbi

감마선처리 냉동 우육, 분쇄우육 및 떡갈비의 판별을 위한 Comet assay의 적용성 평가

  • Jong-Heum Park (Radiation Biotechnology Research Division, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Jae-Kyung Kim (Radiation Biotechnology Research Division, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Beom-Seok Song (Radiation Biotechnology Research Division, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 박종흠 (한국원자력연구원 첨단방사선연구소 방사선바이오연구부) ;
  • 김재경 (한국원자력연구원 첨단방사선연구소 방사선바이오연구부) ;
  • 송범석 (한국원자력연구원 첨단방사선연구소 방사선바이오연구부)
  • Received : 2023.07.28
  • Accepted : 2023.09.06
  • Published : 2023.09.30
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Abstract

This study was conducted to evaluate the applicability of the Comet assay, which is widely used for the identification of irradiated meats, to detect irradiated beef cut, ground beef, and Tteokgalbi during freezing storage. Gamma-irradiation significantly increased the DNA damage in frozen beef cut and ground beef samples. Among those, DNA nuclei of samples irradiated with absorbed doses of 1kGy or more showed typical comet-shaped damage, convincing that the samples were irradiated. Meanwhile, DNA nuclei in non-irradiated beef cut and ground beef samples were also damaged according to storage time. In particular, since the damage of DNA nuclei in the non-irradiated samples frozen for three months was similar to that of samples irradiated with a dose of 0.5 kGy, it was considered difficult to detect whether these samples were irradiated by Comet assay analysis. Likewise, gamma-irradiation of Tteokgalbi increased DNA damage. However, significant damage to DNA nuclei was observed even in the non-irradiated samples. Therefore, the application of the analysis method to determine whether the Tteokgalbi sample was irradiated was not appropriate. In conclusion, these results suggest that Comet assay could be limitedly applied only to fresh meat with a short storage period and minimal processing.

Keywords

Acknowledgement

본 연구는 과학기술정보통신부의 재원으로 한국원자력연구원의 주요사업(523210-23)에 의해 수행되었으며, 그 지원에 감사드립니다.

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