Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
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Effects of Short Microwave Irradiation Time at the Seedlings Stage on the Growth and Secondary Metabolite Contents of Lettuce (Lactuca sativa L.)

유묘단계에서 단시간 마이크로웨이브 처리가 상추의 생육 및 이차대사산물 함량에 미치는 영향

  • Yong Jae Lee (Department of Horticultural Science, Chungnam National University) ;
  • Su Yong Park (Department of Horticultural Science, Chungnam National University) ;
  • Ju Hyung Shin (Department of Bio-AI Convergence, Chungnam National University) ;
  • Seung Yong Hahm (Department of Bio-AI Convergence, Chungnam National University) ;
  • Gwang Ya Lee (Institute of Agricultural Science, Chungnam National University) ;
  • Jong Seok Park (Department of Horticultural Science, Chungnam National University)
  • 이용재 (충남대학교 원예학과) ;
  • 박수용 (충남대학교 원예학과) ;
  • 신주형 (충남대학교 바이오 AI 융합학과) ;
  • 함승용 (충남대학교 바이오 AI 융합학과) ;
  • 이광야 (충남대학교 농업과학연구소) ;
  • 박종석 (충남대학교 농업생명과학대학 원예학과)
  • Received : 2023.01.16
  • Accepted : 2023.07.03
  • Published : 2023.07.31
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Abstract

This experiment was conducted to investigate the effects of microwave irradiation on the growth and secondary metabolite contents of lettuce seedlings. Seedlings at three weeks after sowing were treated by a microwave oven for 0, 4, 8, and 12 seconds with 200 W. After cultivation in a close plant production system for 4 weeks, plant growth measurements and secondary metabolite analysis were performed. The results showed that the fresh and dry weights of the shoot and root, leaf area, leaf length, and the number of leaves were decreased as increasing the microwave treatment times. Chlorophyll a and b, total carotenoids were increased and total phenolics were decreased at the 12-second treatment compared to the 4-second treatment. Total flavonoid contents were decreased at the 8-second treatment compared to the control. These results suggest that the changes in the levels of secondary metabolites were caused by oxidative stress. Although there was no significant difference in secondary metabolite contents excluding total flavonoid contents on the microwave treatments compared to the control, the significant difference suggests that the microwave treatment of 200 W and 2.45 GHz may alter secondary metabolite contents of lettuce after 4 weeks.

본 실험은 마이크로웨이브 처리가 상추 유묘의 생육 변화와 이차대사산물 함량의 변화를 알아보고자 수행되었다. 파종 후 3주째 상추 유묘에 2.45GHz 주파수와 200W의 마이크로웨이브를 0, 4, 8 및 12초 동안 처리하고, 4주간 식물공장에서 재배한 후 생육 및 성분 분석을 수행하였다. 지하부와 지상부의 생체중과 건물중, 엽면적, 엽장 및 엽수는 마이크로웨이브 처리시간이 증가할수록 감소하였다. 4초 처리구와 비교하여 12초 처리구에서 chlorophyll a, chlorophyll b 및 총 carotenoids의 함량이 증가되었으며 총 페놀 함량은 감소하였다. 무처리구와 비교하여 8초 처리구에서 총 플라보노이드 함량이 감소하였다. 이러한 결과들은 산화적 스트레스에 의해 이차대사산물 함량이 변화된 것으로 사료된다. 총 플라보노이드 함량을 제외한 이차대사산물 함량은 각 처리구에서 무처리구와 비교하여 유의한 차이가 없었지만, 각 처리구 사이의 유의한 차이는 200W와 2.45GHz의 마이크로웨이브 처리가 4주 후 상추의 이차대사산물 함량에 영향을 줄 수 있다는 것을 시사한다.

Keywords

Acknowledgement

본 결과물은 농림축산식품부의 재원으로 농림식품기술기획평가원의 농업기반 및 재해대응 기술개발사업의 지원을 받아 연구되었음(322082-3). 2023년도 정부(과학기술정보통신부)의 재원으로 정보통신기획평가원의 지원을 받아 수행된 연구임(No.RS-2022-00155857, 인공지능융합혁신인재양성(충남대학교)).

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