대한수의학회지 (Korean Journal of Veterinary Research)

  • 제63권2호
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  • Pages.18.1-18.8
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  • 2023
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  • 2466-1384(pISSN)
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  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지
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Porcine epidemic diarrhea viruses from Vietnam: isolation, characterization, and neutralizing activity

  • Van Tan Do (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Quang Lam Truong (Key Laboratory of Veterinary Biotechnology, Faculty of Veterinary Medicine, Vietnam National University of Agriculture) ;
  • Hoai Thu Dao (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University) ;
  • Thi Lan Nguyen (Key Laboratory of Veterinary Biotechnology, Faculty of Veterinary Medicine, Vietnam National University of Agriculture) ;
  • Minna Shin (INNOVAC) ;
  • Kyeong Ryeol Shin (INNOVAC) ;
  • Tae-Wook Hahn (College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University)
  • 투고 : 2023.04.11
  • 심사 : 2023.06.08
  • 발행 : 2023.06.30
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초록

Porcine epidemic diarrhea (PED) is characterized by acute enteritis, watery diarrhea, weight loss, dehydration, and death, with high mortality in neonatal piglets. In this study, 3 virus isolates collected in Vietnam between 2016 and 2017 were propagated successfully in Vero cells at high virus titers. Sequence analysis of the full-length spike (S) gene showed that all 3 isolates belong to genogroup 2b, which is closely related to other prevalent Asian strains. A comparison of the amino acid sequence revealed a 98.19% to 99.13% homology with the Vietnam isolates circulating during 2013-2015, suggesting that field PED viruses (PEDVs) are evolving continuously. Experiments in animals showed that the antisera from guinea pigs immunized with the vaccine strain resulted in higher levels (5 log2) of neutralizing antibodies against the homologous strain and a relatively moderate level of neutralizing antibodies against the field isolates. This finding would be helpful in selecting a PEDV strain for vaccine development.

키워드

과제정보

The authors wish to acknowledge the veterinarians who collected the infected samples and the contribution of all authors for this study.

참고문헌

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