Korean Circulation Journal

  • 제53권1호
  • /
  • Pages.34-46
  • /
  • 2023
  • /
  • 1738-5520(pISSN)
  • /
  • 1738-5555(eISSN)
대한심장학회 (The Korean Society of Cardiology)
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Development of a Zebrafish Larvae Model for Diabetic Heart Failure With Reduced Ejection Fraction

  • Inho Kim (Department of Internal Medicine, Seoul National University Hospital) ;
  • Seung Hyeok Seok (Department of Microbiology and Immunology, Seoul National University College of Medicine) ;
  • Hae-Young Lee (Department of Internal Medicine, Seoul National University Hospital)
  • 투고 : 2022.08.01
  • 심사 : 2022.11.09
  • 발행 : 2023.01.01
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초록

Background and Objectives: Diabetes mellitus (DM)-associated heart failure (HF) causes high morbidity and mortality. In this study, we established a zebrafish larvae model for in vivo research on diabetic HF. Methods: DM-like phenotypes were induced by treating zebrafish larvae with a combination of D-glucose (GLU) and streptozotocin (STZ). HF was induced by treatment with terfenadine (TER), a potassium channel blocker. Additionally, myocardial contractility, motility, and viability were evaluated. Results: The zebrafish larvae treated with a combination of GLU and STZ showed significantly higher whole-body glucose concentrations, lower insulin levels, and higher phosphoenolpyruvate carboxykinase levels, which are markers of abnormal glucose homeostasis, than the group treated with only GLU, with no effect on viability. When treated with TER, DM zebrafish showed significantly less myocardial fractional shortening and more irregular contractions than the non-DM zebrafish. Furthermore, in DM-HF with reduced ejection fraction (rEF) zebrafish, a significant increase in the levels of natriuretic peptide B, a HF biomarker, markedly reduced motility, and reduced survival rates were observed. Conclusions: We established a DM-HFrEF zebrafish model by sequentially treating zebrafish larvae with GLU, STZ, and TER. Our findings indicate the potential utility of the developed zebrafish larvae model not only in screening studies of new drug candidates for DM-HFrEF but also in mechanistic studies to understand the pathophysiology of DM-HFrEF.

키워드

과제정보

This work was supported by a National Research Foundation (NRF) grant funded by the Korean government (2020R1A2C2010202).

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