Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
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Assessment of In vitro Antioxidant, Antidiabetic and Cytotoxic Activities of Sphaeranthus africanus Extracts

  • Tran Thi Huyen (Research Center for Genetics and Reproductive Health – CGRH, School of Medicine, Vietnam National University HCM City) ;
  • Julien Dujardin (Research Center of Ginseng and Medicinal Materials Ho Chi Minh City 2) ;
  • Nguyen Thi Thu Huong (Research Center of Ginseng and Medicinal Materials Ho Chi Minh City 2) ;
  • Chung Thi My Duyen (Research Center of Ginseng and Medicinal Materials Ho Chi Minh City 2) ;
  • Nguyen Hoang Minh (Research Center of Ginseng and Medicinal Materials Ho Chi Minh City 2) ;
  • Ha Quang Thanh (Research Center of Ginseng and Medicinal Materials Ho Chi Minh City 2) ;
  • Dao Tran Mong (Research Center of Ginseng and Medicinal Materials Ho Chi Minh City 2) ;
  • Ly Hai Trieu (Research Center of Ginseng and Medicinal Materials Ho Chi Minh City 2) ;
  • Nguyen Mai Truc Tien (Research Center of Ginseng and Medicinal Materials Ho Chi Minh City 2) ;
  • Mai Thanh Chung (Research Center of Ginseng and Medicinal Materials Ho Chi Minh City 2) ;
  • Nguyen Nhat Minh (Research Center of Ginseng and Medicinal Materials Ho Chi Minh City 2) ;
  • Nguyen Thi Ngoc Dan (Research Center of Ginseng and Medicinal Materials Ho Chi Minh City 2) ;
  • Huynh Loi (Faculty of Pharmacy, Binh Duong University)
  • Received : 2023.03.02
  • Accepted : 2023.06.02
  • Published : 2023.06.30
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Abstract

Sphaeranthus africanus is commonly used as a traditional remedy for sore throats and pain treatment in Vietnam. The aerial parts have been studied for its anti-inflammatory and anti-proliferative properties. However, the antioxidant and antidiabetic potential of the plant has not been explored. In this work, hydrophilic extracts of the plant's aerial parts were prepared in order to investigate its antioxidant and anti-diabetic properties. Also, the cytotoxicity of the root was evaluated and compared to that of the aerial parts. All of the extracts inhibited lipid peroxidation with IC50 values ranging from 2.05 to 3.56 ㎍/mL, indicating substantial antioxidant activity. At an IC50 value of 4.80 ㎍/mL, the 50% ethanol extract exhibited the most potent inhibition of α-glucosidase. The cytotoxic activity of root extracts is 2 to 5-fold less than that of the aerial parts. Nevertheless, dichloromethane and ethyl acetate extracts of the root demonstrated a selective effect on leukemia cells, with no harm towards the normal HEK-293 cell line. This work provides a scientific support for the antioxidant and antidiabetic activity of the plant. Hence, it may find a promising material for the development of novel antioxidant and antidiabetic agents. More research can be conducted on the phytochemistry and anticancer activities of the plant's root.

Keywords

Acknowledgement

This research was funded by the Vietnam National University Hochiminh city (Project No. C2022-44-01). The authors wish to thank the Department of Pharmacognosy, University of Graz, for assistance with cytotoxic protocol.

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