Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Direct Monitoring of Membrane Fatty Acid Changes and Effects on the Isoleucine/Valine Pathways in an ndgR Deletion Mutant of Streptomyces coelicolor

  • Tae-Rim Choi (Department of Microbial Engineering, College of Engineering, Konkuk University) ;
  • Suk Jin Oh (Department of Microbial Engineering, College of Engineering, Konkuk University) ;
  • Jeong Hyeon Hwang (Department of Microbial Engineering, College of Engineering, Konkuk University) ;
  • Hyun Jin Kim (Department of Microbial Engineering, College of Engineering, Konkuk University) ;
  • Nara Shin (Department of Microbial Engineering, College of Engineering, Konkuk University) ;
  • Jeonghee Yun (Department of Forest Products and Biotechnology, Kookmin University) ;
  • Sang-Ho Lee (Department of Pharmacy, College of Pharmacy, Jeju National University) ;
  • Shashi Kant Bhatia (Department of Microbial Engineering, College of Engineering, Konkuk University) ;
  • Yung-Hun Yang (Department of Microbial Engineering, College of Engineering, Konkuk University)
  • Received : 2023.01.12
  • Accepted : 2023.03.14
  • Published : 2023.06.28
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Abstract

NdgR, a global regulator in soil-dwelling and antibiotic-producing Streptomyces, is known to regulate branched-chain amino acid metabolism by binding to the upstream region of synthetic genes. However, its numerous and complex roles are not yet fully understood. To more fully reveal the function of NdgR, phospholipid fatty acid (PLFA) analysis with gas chromatography-mass spectrometry (GC-MS) was used to assess the effects of an ndgR deletion mutant of Streptomyces coelicolor. The deletion of ndgR was found to decrease the levels of isoleucine- and leucine-related fatty acids but increase those of valine-related fatty acids. Furthermore, the defects in leucine and isoleucine metabolism caused by the deletion impaired the growth of Streptomyces at low temperatures. Supplementation of leucine and isoleucine, however, could complement this defect under cold shock condition. NdgR was thus shown to be involved in the control of branched-chain amino acids and consequently affected the membrane fatty acid composition in Streptomyces. While isoleucine and valine could be synthesized by the same enzymes (IlvB/N, IlvC, IlvD, and IlvE), ndgR deletion did not affect them in the same way. This suggests that NdgR is involved in the upper isoleucine and valine pathways, or that its control over them differs in some respect.

Keywords

Acknowledgement

This study was supported by the Research Program to Solve Social Issues with the National Research Foundation of Korea (NRF), funded by the Ministry of Science and ICT [Grant No. 2017M3A9E4077234], National Research Foundation of Korea (NRF) [Grant Nos. NRF-2022M3I3A1082545, NRF-2022R1A2C2003138]. This study was also supported by the R&D Program of MOTIE/KEIT [Grant Nos. 20009508 and 20014350]. The authors also acknowledge the KU Research Professor Program of Konkuk University, Seoul, South Korea.

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