Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Quercetin Attenuates the Production of Pro-Inflammatory Cytokines in H292 Human Lung Epithelial Cells Infected with Pseudomonas aeruginosa by Modulating ExoS Production

  • Hye In Ahn (Life Science Research Center, Nine Biopharm Co., LTD) ;
  • Hyun-Jae Jang (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ok-Kyoung Kwon (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jung-Hee Kim (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Jae-Hoon Oh (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Seung-Ho Kim (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Sei-Ryang Oh (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Sang-Bae Han (College of Pharmacy, Chungbuk National University) ;
  • Kyung-Seop Ahn (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Ji-Won Park (Natural Medicine Research Center, Korea Research Institute of Bioscience and Biotechnology)
  • Received : 2022.08.23
  • Accepted : 2023.01.11
  • Published : 2023.04.28
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Abstract

The type three secretion system (T3SS) is a major virulence system of Pseudomonas aeruginosa (P. aeruginosa). The effector protein Exotoxin S (ExoS) produced by P. aeruginosa is secreted into the host cells via the T3SS. For the purpose of an experiment on inhibitors with regard to ExoS secretion, we developed a sandwich-type enzyme-linked immunosorbent assay (ELISA) system. Quercetin was selected because it has a prominent ExoS inhibition effect and also is known to have anti-inflammatory and antioxidant effects on mammalian cells. In this study, we investigated the effects of quercetin on the expression and secretion of ExoS using ELISA and Western blot analysis methods. The results showed that the secretion of ExoS was significantly decreased by 10 and 20 µM of quercetin. Also, popB, popD, pscF, and pcrV which are composed of the T3SS needle, are reduced by quercetin at the mRNA level. We also confirmed the inhibitory effect of quercetin on cytokines (IL-6, IL-1β, and IL-18) in P. aeruginosa-infected H292 cells by real-time polymerase chain reaction (PCR) and ELISA. Collectively, quercetin inhibits the secretion of ExoS by reducing both ExoS production and the expression of the needle protein of T3SS. Furthermore, these results suggest that quercetin has the potential to be used as an anti-toxic treatment for the inflammatory disease caused by P. aeruginosa infection.

Keywords

Acknowledgement

This study was supported by the Bio and Medical Technology Development Program of the National Research Foundation (NRF) and funded by the Korean government (grant number: NRF-2020R1A2C2101228) and awarded to the Korea Research Institute of Bioscience and Biotechnology(KRIBB) Research Initiative Program (KGM5522322) of the Republic of Korea. The funders had no role in data analysis, study design or preparation of the manuscript.

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