Journal of the Society of Cosmetic Scientists of Korea (대한화장품학회지)

Society of Cosmetic Scientists of Korea (대한화장품학회)
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Effect of Skin Elasticity Improvement and Anti-oxidant Activity of Stem Cells Extract Derived from Cambium of Aloe

알로에 형성층 유래 줄기세포 추출물의 항산화 활성 및 피부탄력 개선 효과

  • 김동명 ((주)케이제이엠바이오 연구소) ;
  • 김원진 (KAIST 생명과학과) ;
  • 이형곤 ((주)케이제이엠바이오 연구소) ;
  • 권용성 ((주)케이제이엠바이오 연구소) ;
  • 최연매 ((주)김정문알로에)
  • Received : 2023.05.24
  • Accepted : 2023.06.30
  • Published : 2023.06.30
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Abstract

In this study, stem cells were extracted from the callus derived from the aloe cambium, and the antioxidant activity and effect of skin elasticity improvement were assessed. The aloe cambium-derived stem cell extract, AloStem and callus showed the survival rate of each 98.27% and 71.31%. In the results of the DPPH antioxidant activity of AloStem and aloe extract, it was confirmed that the antioxidant effect of AloStem was more than twice that of Aloe extract. AloStem did not affect the cytotoxicity of normal human dermal fibroblasts (NHDF) cells up to 0.25% concentration. Also, AloStem increased elastin, COL1A1 and HAS2 mRNA expression levels dose-dependently. Furthermore, to examine lifting effect of skin elasticity using a sheet mask containing AloStem, 21 adult men and women applied the sheet mask in the face, once a day for 2 weeks. As a result, after 2 weeks the skin length was 116.75 ± 5.58 mm before the use of the sheet mask, but after 2 weeks of use, it was confirmed that 0.59% increased to 117.44 ± 5.17 mm. Thus, we concluded that the sheet mask containing AloStem can help the lifting effect of skin elasticity.

본 연구는 알로에 형성층으로부터 유도된 캘러스에서 줄기세포를 추출하고, 이에 대한 항산화 활성 및 피부 탄력 개선효과를 확인하고자 하였다. 알로에 형성층 유래 줄기세포 추출물 AloStem과 캘러스의 세포 생존율을 확인한 결과, AloStem은 98.27%, 캘러스는 71.31%의 세포가 생존하는 것으로 확인되었다. AloStem의 DPPH 항산화 활성을 측정한 결과, 알로에 추출물보다 AloStem에서 2 배 이상의 항산화 효과를 가지는 것으로 확인되었다. AloStem이 함유된 겔에 대한 인간진피섬유아세포의 독성을 확인한 결과, 0.25% 농도까지 영향을 미치지 않았으며, elastin, COL1A1 및 HAS2 mRNA 발현량을 증가시켰다. 또한, AloStem이 함유된 시트마스크를 이용하여 피부탄력 리프팅 효과에 대한 임상적 연구를 성인남녀 21 명을 대상으로 2 주간 진행한 결과, 피부 길이가 시트마스크 사용 전에는 116.75 ± 5.58 mm로 나타났으나, 2 주 사용 후에는 117.44 ± 5.17 mm 로 0.59%가 증가된 것으로 확인되어 AloStem을 함유한 시트마스크는 탄력 개선에 의한 리프팅 효과에 도움을 주는 것으로 최종 확인되었다.

Keywords

Acknowledgement

본 논문은 2021년도 중소벤처기업부의 재원으로 창업성장기술개발사업의 지원을 받아 수행된 연구이다(과제번호: S3049433).

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