The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Anti-inflammatory Effect of Eleutherococcus senticosus Extracts from Gangwon-do by Plant parts or Solvents

강원도 가시오갈피의 식물 부위 또는 추출 용매 조건에 따른 항염증 효과

  • Junkyu Park (Department of Science in Korean Medicine, College of Korean Medicine, Graduate School, Kyung Hee University) ;
  • Mina Boo (Department of Science in Korean Medicine, College of Korean Medicine, Graduate School, Kyung Hee University) ;
  • Soojin An (College of Korean Medicine, Kyung Hee University) ;
  • Sujin Shin (Department of Korean Medicine, College of Korean Medicine, Kyung Hee University) ;
  • Jinbong Park (Department of Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Ho-Young Choi (Department of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University) ;
  • Kyungjin Lee (Department of Herbal Pharmacology, College of Korean Medicine, Kyung Hee University)
  • 박준규 (경희대학교 대학원 한의과대학 기초한의과학과) ;
  • 부민아 (경희대학교 대학원 한의과대학 기초한의과학과) ;
  • 안수진 (경희대학교 한의과대학 한의학과) ;
  • 신수진 (경희대학교 한의과대학 한의학과) ;
  • 박진봉 (경희대학교 한의과대학 약리학교실) ;
  • 최호영 (경희대학교 한의과대학 본초학교실) ;
  • 이경진 (경희대학교 한의과대학 본초학교실)
  • Received : 2023.05.26
  • Accepted : 2023.07.25
  • Published : 2023.07.30
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Abstract

Background : The purpose of this study was to investigate the anti-inflammatory properties of stems and leaves of Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. (ES) from Gangwon-do. Methods and Results : Stems and leaves of ES were collected from two areas in Gangwon-do: Cheorwon-gun and Samcheok-si. Samples were extracted with water by using the pressurized liquid extraction method and with 70% prethanol A by using the heat reflux extraction method. The anti-inflammatory effects of ES were evaluated through the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide(MTT), lactate dehydrogenase(LDH) assay, nitric oxide(NO) assay, enzyme-linked immunosorbent assay(ELISA), and Western blot analysis in RAW 264.7 macrophages stimulated with lipopolysaccharide (LPS). 1) Results showed that ES leaf extractions were not cytotoxic at a concentration of up to 30 ㎍/㎖. The leaves of 70% prethanol A extractions of ES(30 ㎍/㎖) inhibited NO, interleukin-6(IL-6), and tumor necrosis factor-α(TNF-α) production and decreased the protein level of cyclooxygenase 2(COX-2). There was no significant change in the protein level of inducible nitric oxide synthase(iNOS). The stem extractions of ES did not exhibit anti-inflammatory effects. Conclusions : In this study, the leaves of 70% prethanol A extractions of ES demonstrated anti-inflammatory effect on RAW 264.7 macrophages. The 70% prethanol A extractions have a relatively higher anti-inflammatory effect on RAW 264.7 macrophages than water extractions.

Keywords

Acknowledgement

본 성과물(논문)은 농촌진흥청 연구사업(세부과제번호: PJ01704503)의 지원에 의해 이루어진 것임.

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