Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Characterization of L-(+)-Lactic Acid Producing Weizmannia coagulans Strains from Tree Barks and Probiogenomic Evaluation of BKMTCR2-2

  • Jenjuiree Mahittikon (Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University) ;
  • Sitanan Thitiprasert (Center of Excellence in Bioconversion and Bioseparation for Platform Chemical Production, Institute of Biotechnology and Genetic Engineering, Chulalongkorn University) ;
  • Sitanan Thitiprasert (Center of Excellence in Bioconversion and Bioseparation for Platform Chemical Production, Institute of Biotechnology and Genetic Engineering, Chulalongkorn University) ;
  • Naoto Tanaka (Department of Molecular Microbiology, Faculty of Life Sciences, Tokyo University of Agriculture) ;
  • Yuh Shiwa (Department of Molecular Microbiology, Faculty of Life Sciences, Tokyo University of Agriculture) ;
  • Nitcha Chamroensaksri (National Biobank of Thailand (NBT), National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)) ;
  • Somboon Tanasupawat (Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Sciences, Chulalongkorn University)
  • Received : 2023.08.21
  • Accepted : 2023.10.06
  • Published : 2023.12.28
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Abstract

This study aimed to isolate and identify L-(+)-lactic acid-producing bacteria from tree barks collected in Thailand and evaluate the potential strain as probiotics. Twelve strains were isolated and characterized phenotypically and genotypically. The strains exhibited a rod-shaped morphology, high-temperature tolerance, and the ability to ferment different sugars into lactic acid. Based on 16S rRNA gene analysis, all strains were identified as belonging to Weizmannia coagulans. Among the isolated strains, BKMTCR2-2 demonstrated exceptional lactic acid production, with 96.41% optical purity, 2.33 g/l of lactic acid production, 1.44 g/g of lactic acid yield (per gram of glucose consumption), and 0.0049 g/l/h of lactic acid productivity. This strain also displayed a wide range of pH tolerance, suggesting suitability for the human gastrointestinal tract and potential probiotic applications. The whole-genome sequence of BKMTCR2-2 was assembled using a hybridization approach that combined long and short reads. The genomic analysis confirmed its identification as W. coagulans and safety assessments revealed its non-pathogenic attribute compared to type strains and commercial probiotic strains. Furthermore, this strain exhibited resilience to acidic and bile conditions, along with the presence of potential probiotic-related genes and metabolic capabilities. These findings suggest that BKMTCR2-2 holds promise as a safe and effective probiotic strain with significant lactic acid production capabilities.

Keywords

Acknowledgement

This research was supported by the Development and Promotion of Science and Technology Talents Project (DPST), Thai government scholarship as a scholarship to Jenjuiree M. (561060), the 90th Anniversary of Chulalongkorn University Fund (Ratchadaphiseksomphot Endowment Fund), Graduate School, Chulalongkorn University, and the Faculty of Pharmaceutical Sciences, Chulalongkorn University for providing research fund (Grant number Phar2565-RG002) to Dr. Somboon Tanasupawat. The genomic analysis was associated by the Department of Molecular Microbiology, Tokyo University of Agriculture. The authors thank the Pharmaceutical Research Instrument Center, Faculty of Pharmaceutical Sciences, Chulalongkorn University for providing research facilities; Dr. Engkarat Kingkaew; Dr. Sukanya Phuengjayaem, Dr. Saranporn Poothong and all friends for consistency encouragement to pass through the research project.

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