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Complete Genome Sequence of an optrA-positive Linezolid-resistant Staphylococcus rostri Strain PJFA-333 Isolated from a Pig in Korea

  • Gi Yong Lee (Department of Veterinary Microbiology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University) ;
  • Soo-Jin Yang (Department of Veterinary Microbiology, College of Veterinary Medicine and Research Institute for Veterinary Science, Seoul National University)
  • Received : 2023.08.16
  • Accepted : 2023.09.20
  • Published : 2023.09.28

Abstract

Linezolid, the first oxazolidinone introduced into human clinical use, has become a last resort antibiotic in treatment of serious infections caused by Gram-positive pathogens, including methicillin-resistant staphylococci and vancomycin-resistant enterococci. Although oxazolidinones are strictly prohibited for use in food-producing animals, occurrence of linezolid-resistant staphylococci has recently been reported in livestock farms in Korea. Here, we report the complete genome sequence of an optrA-positive linezolid-resistant Staphylococcus rostri strain PJFA-333 isolated from a pig farm in Korea.

Keywords

Acknowledgement

This study was supported by grants from Research of Korea Centers for Disease Control and Prevention (2020ER540500) and Ministry of Food and Drug Safety (23194MFDS012).

References

  1. Lee GY, Kim GB, Yang SJ. 2022. Co-occurrence of cfr-mediated linezolid-resistance in ST398 LA-MRSA and non-aureus staphylococci isolated from a pig farm. Vet. Microbiol. 266: 109336. 
  2. Schwarz S, Werckenthin C, Kehrenberg C. 2000. Identification of a plasmid-borne chloramphenicol-florfenicol resistance gene in Staphylococcus sciuri. Antimicrob. Agents Chemother. 44: 2530-2533.  https://doi.org/10.1128/AAC.44.9.2530-2533.2000
  3. Wang Y, Lv Y, Cai J, Schwarz S, Cui L, Hu Z, et al. 2015. A novel gene, optrA, that confers transferable resistance to oxazolidinones and phenicols and its presence in Enterococcus faecalis and Enterococcus faecium of human and animal origin. J. Antimicrob. Chemother. 70: 2182-2190.  https://doi.org/10.1093/jac/dkv116
  4. Overbeek R, Olson R, Pusch GD, Olsen GJ, Davis JJ, Disz T, et al. 2014. The SEED and the Rapid Annotation of microbial genomes using Subsystems Technology (RAST). Nucleic Acids Res. 42: D206-214.  https://doi.org/10.1093/nar/gkt1226
  5. Seemann T. 2014. Prokka: rapid prokaryotic genome annotation. Bioinformatics 30: 2068-2069.  https://doi.org/10.1093/bioinformatics/btu153
  6. Yoon SH, Ha SM, Lim J, Kwon S, Chun J. 2017. A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie Van Leeuwenhoek 110: 1281-1286.  https://doi.org/10.1007/s10482-017-0844-4
  7. Clinical and Laboratory Standards Institute (CLSI). 2022. Performance Standards for Antimicrobial Susceptibility Testing M100 32th edition.