한국해양바이오학회지 (Journal of Marine Bioscience and Biotechnology)

한국해양바이오학회 (The Korean Society for Marine Biotechnology)
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갯완두(Lathyrus japonicus) 에탄올 추출물의 항산화 및 항염증 효능

Antioxidant and anti-inflammatory activities of Lathyrus japonicus ethanol extracts

  • Yun Gyeong Park (National Marine Biodiversity Institute of Korea) ;
  • Myeong Seok Lee (National Marine Biodiversity Institute of Korea) ;
  • Seok-Chun Ko (National Marine Biodiversity Institute of Korea) ;
  • Jeong Min Lee (National Marine Biodiversity Institute of Korea) ;
  • Mi-Jin Yim (National Marine Biodiversity Institute of Korea) ;
  • Kyung Woo Kim (National Marine Biodiversity Institute of Korea) ;
  • Hyun-Soo Kim (National Marine Biodiversity Institute of Korea) ;
  • Ji-Yul Kim (National Marine Biodiversity Institute of Korea) ;
  • Chul Hwan Kim (National Marine Biodiversity Institute of Korea) ;
  • Jung Hye Won (National Marine Biodiversity Institute of Korea) ;
  • Dae-Sung Lee (National Marine Biodiversity Institute of Korea) ;
  • Gun-Woo Oh (National Marine Biodiversity Institute of Korea)
  • 투고 : 2023.03.04
  • 심사 : 2023.03.13
  • 발행 : 2023.06.30
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초록

In the present study, the antioxidant and anti-inflammatory activities of ethanolic extracts from Lathyrus japonicus at concentrations of 50, 100, and 200 ㎍/mL were investigated in LPS-stimulated, RAW264.7 cells. Antioxidant properties were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging assays and ferric reducing antioxidant power assay. In addition, the production of reactive oxygen species (ROS) was measured using the 2',7'-dichlorofluorescein diacetate (DCFH-DA) probe by flow cytometry. To examine the anti-inflammatory activity of the extracts of L. japonicus, their effects on the levels of nitric oxide (NO); production of cytokines such as interleukin (IL)-1β, IL-10, and tumor necrosis factor-α (TNF-α); and the activities of enzymes such as inducible NOS (iNOS) and cyclooxygenase-2 (COX-2) were assessed. The IC50 values of the DPPH and ABTS radical scavenging assays were 476.09 ± 1.50 and 34.91 ± 0.37 ㎍/mL, respectively. In addition, L. japonicus extracts not only inhibited ROS production, but also the production of NO, IL-1β, and IL-10, and the activity of iNOS in a dose-dependent manner. In summary, the ethanolic extracts of L. japonicus could be used as a functional food additive and an anti-inflammatory agent owing to their antioxidant and anti-inflammatory activities

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과제정보

이 논문은 2023년 국립해양생물자원관 재원으로 지원을 받아 수행된 연구임(2023M00500).

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