Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지
DOI QR코드

DOI QR Code

Residue analysis of florfenicol in cultured eel Anguilla japonica following oral administration

양식 뱀장어(Anguilla japonica)에서 florfenicol의 경구투여에 따른 잔류 연구

  • A Reum Kim (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Dong Hun Shin (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Jun Sung Bae (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Kang Uk Lee (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Chae Won Lee (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Chan Yeong Yang (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Eun Ha Jeong (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University) ;
  • Ji-Hoon Lee (National Fishery Products Quality Management Service) ;
  • Su-Jin Bak (National Fishery Products Quality Management Service) ;
  • Kwan Ha Park (Department of Aquatic Life Medicine, College of Ocean Science and Technology, Kunsan National University)
  • 김아름 (군산대학교 해양과학대학 수산생명의학과) ;
  • 신동훈 (군산대학교 해양과학대학 수산생명의학과) ;
  • 배준성 (군산대학교 해양과학대학 수산생명의학과) ;
  • 이강욱 (군산대학교 해양과학대학 수산생명의학과) ;
  • 이채원 (군산대학교 해양과학대학 수산생명의학과) ;
  • 양찬영 (군산대학교 해양과학대학 수산생명의학과) ;
  • 정은하 (군산대학교 해양과학대학 수산생명의학과) ;
  • 이지훈 (국립수산물품질관리원) ;
  • 박수진 (국립수산물품질관리원) ;
  • 박관하 (군산대학교 해양과학대학 수산생명의학과)
  • Received : 2023.04.27
  • Accepted : 2023.06.01
  • Published : 2023.06.30
Download PDF
⟨ Previous Next ⟩

Abstract

Florfenicol (FF) is a member of chloramphenicol (CM) family member of antibiotics developed to take advantage of not causing aplastic anemia, the typical side effect of CM. Because FF is a safe veterinary drug it is also approved for clinical use for fish in Korea. The usage, dose and with-drawal period of FF in Korea are the same as in Japan, but the maximum residue limit (MRL) is set differently for eel, 0.2 mg/kg in Korea and 8.0 mg/kg in Japan. However, there is a lack of research on residue characteristics that meets domestic standards to support this claim. In this study, we conducted tests in order to set a drug withdrawal period in cultured eel Anguilla japonica. Following administrate of FF up to 20 mg/kg, twice the approved clinical dose of 10 mg/kg for 5-days, it is residual was evaluated. Trend. It was found that the previously set 7-day withdrawal period needs to be extended to approximatiy to 60 days. The results of this study were used as evidence for the recent year 2022 extension of the withdrawal period from 7 to 60 days.

Florfenicol (FF)은 chloramphenicol(CM)계의 항생제로 CM의 대표적 부작용인 재생불량성 빈혈을 유발하지 않는 장점을 활용하기 위해 개발되었다. FF은 세계적으로 안전하게 사용중인 동물용의약품 중 하나로 우리나라도 어류에게 임상적으로의 사용이 허가된 항생제이다. 우리나라의 FF의 용법, 용량 및 휴약기간은 일본과 설정이 동일하다 하지만 최대잔류허용기준(maximum residue limit, MRL)은 0.2 mg/kg과 8.0 mg/kg으로 상이하게 설정되어있다. 하지만 이를 뒷받침할 국내 기준에 맞는 잔류량 특성 연구가 미비한 실정이다. 본 연구는 식품안전을 목적으로 휴약기간을 설정하기 위하여 농림축산검역본부에서 고시한 동물용의약품 잔류성 시험지침에 따라 시험을 진행하였으며, 양식 뱀장어인 Anguilla japonica에서 FF의 잔류 양상을 분석하였다. FF을 5일간 반복투여 방법으로 승인된 임상 용량인 10 mg/kg과 2배인 20 mg/kg까지 투여하였으며, 잔류경향을 평가한 결과 이전에 설정되어있던 7일의 휴약기간을 약 60일로 연장할 필요가 있는 것으로 밝혀졌다. 이 연구의 결과는 이전에 7일이었던 휴약기간을 최근(2022년) 60일로 연장하는데 근거자료로 활용된 바 있다.

Keywords

References

  1. Dowling P.M.: Chloramphenicol, thiamphenicol, and florfenicol. Antimicrobial Therapy in Veterinary Medicine, 269-277, 2013. 
  2. Ellis, A.E.: The anatomy and physiology of teleosts. Fish pathology, 13-54, 1978. 
  3. Horsberg T.E., Martinsen, B. and Varma, K.J.: The disposition of 14C-florfenicol in Atlantic salmon (Salmo salar). Aquaculture, 122(2-3): 97-106, 1994.  https://doi.org/10.1016/0044-8486(94)90502-9
  4. Horsberg T.E., Hoff K.A. and Nordmo R.: Pharmacokinetics of florfenicol and its metabolite florfenicol amine in atlantic Salmon. Journal of Aquatic Animal Health, 8(4): 292-301, 1996.  https://doi.org/10.1577/1548-8667(1996)008<0292:POFAIM>2.3.CO;2
  5. Kim Y.J., Seo J.S., Park J.O., Jeong A.R. and Lee J.H.: Monitoring of aquatic medicine managements in South Korea. Journal of Fish Pathology, 32(1): 37-43, 2019.  https://doi.org/10.7847/JFP.2019.32.1.037
  6. Lin, M., Fan, H., Chen, Z. and Yao, Z.: Pharmacokinetics of florfenicol in Japanese eel (Anguilla japonica) after a single oral dose. International Symposium on Information Technologies in Medicine and Education, Vol. 2, 813-815, 2012. 
  7. Lin, M., Chen, Z., Ji, R., Yang, X. and Wang, J.: Comparative pharmacokinetics of florfenicol in Japanese eels at different temperature. Journal of Shanghai Ocean University, 22(2): 225-231, 2013. 
  8. MFDS (Ministry of Food and Drug Safety) : Food code. https://various.foodsafetykorea.go.kr/fsd/#/ext/Document/FC?searchNm=florfenicol&itemCode=FC0A074001002A169 
  9. MFDS (Ministry of food and drug safety): MINISTRY OF FOOD AND DRUG SAFETY, pp. 189, pp. 274, pp. 352, pp. 483, Korea, 2020. 
  10. MOF (Ministry of Oceans and Fisheries) : 내수면어업주요 품종별,연도별 생산량. https://www.mof.go.kr/statPortal/cate/partStat.do 
  11. NFQS (National Fishery Products Quality Management Service): Aquatic Medicine Catalog, pp. 53-68, Korea, 2022. 
  12. NIFS (National Institute of Fisheries Science): Antibiotics for fish. https://www.nifs.go.kr/page?id=antibiotics_1_05, 2022. 
  13. Park B.K., Lim J.H., Kim M.S. and Yun H.I.: Pharmacokinetics of florfenicol and its metabolite, florfenicol amine, in the Korean catfish (Silurus asotus). Journal of Veterinary Pharmacology and Therapeutics, 29(1): 37-40, 2006.  https://doi.org/10.1111/j.1365-2885.2006.00709.x
  14. Seo J.S and Jung S.H.: Antimicrobial activity of florfenicol against fish pathogenic bacteria and pharmacokinetics in blood of cultured olive flounder by oral administration. Journal of Fishries and Marine Sciences, 25(5): 1079-1087, 2013. 
  15. Seo J.S and Jung S.H.: Pharmacokinetics of florfenicol in blood of the culture korean rockfish following single oral administration. Journal of Fishries and Marine Sciences, 32(3): 694-700, 2020.