한국어병학회지 (Journal of fish pathology)

  • 제36권1호
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  • Pages.107-116
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  • 2023
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  • 1226-0819(pISSN)
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  • 2233-5412(eISSN)
한국어병학회 (The Korean Society of Fish Pathology)
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양식 극동산 뱀장어(Anguilla japonica)에서 분리된 Lactococcus lactis의 항생제 내성

The antibiotic resistance of Lactococcus lactis isolated from the farmed Anguilla japonica

  • 김현우 (선문대학교 수산생명의학과) ;
  • 이은섭 (선문대학교 수산생명의학과) ;
  • 이승준 (선문대학교 수산생명의학과) ;
  • 한소라 (유전체 기반 바이오IT 융합연구소) ;
  • 오태진 (선문대학교 제약생명공학과) ;
  • 김명석 (국립수산과학원 병리연구과) ;
  • 김수진 (국립수산과학원 병리연구과) ;
  • 권세련 (선문대학교 수산생명의학과)
  • Hyunwoo Kim (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • Eunsup Lee (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • Sung Jun Lee (Department of Aquatic Life Medical Sciences, Sunmoon University) ;
  • So-Ra Han (Genome-based BioIT Convergence Institute) ;
  • Tae-Jin Oh (Department of Pharmaceutical Engineering and Biotechnology, Sunmoon University) ;
  • Myoung Sug Kim (Pathology Research Division, National Institute of Fisheries Science) ;
  • Soo-Jin Kim (Pathology Research Division, National Institute of Fisheries Science) ;
  • Se Ryun Kwon (Department of Aquatic Life Medical Sciences, Sunmoon University)
  • 투고 : 2023.05.23
  • 심사 : 2023.06.05
  • 발행 : 2023.06.30
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초록

Spleen, liver and intestinal mucus of Anguilla japonica cultured in a domestic recirculating aquaculture system were sampled to isolate and identify culturable bacteria. Based on 16s rRNA sequence analysis, the most frequently isolated bacterium was Lactococcus lactis. To determine the antibiotic susceptibility of L. lactis, the minimum inhibitory concentration (MIC) test was conducted using five isolates (A1, A21, A65, A125 and A130) from Anguilla japonica and three isolates (KCTC2013, KCTC3769 and KCTC3899) from Korean Collection for Type Culture. Differences of MICs between A. japonica-derived isolates and KCTC isolates were observed in Tetracycline, Oxytetracycline, Doxycyclin and Erythromycin. MICs obtained for five L. lactis isolates from A. japonica were >64 ㎍/mL for Tetracycline, >256 ㎍/mL for Oxytetracycline, 16-32 ㎍/mL for Doxycyclin, and > 64㎍/mL for Erythromycin. On the other hand, KCTC isolates showed the MICs of 0.25 ㎍/mL, 0.25 ㎍/mL, 0.12 ㎍/mL and 0.03-0.06 ㎍/mL for respective antibiotics. Furthermore, it was confirmed that genomic DNA and plasmid DNA isolated from A. japonica-derived L. lactis isolates have antibiotic resistance genes and transfer origin. These findings suggest that A. japonica-derived L. lactis isolates may have the potential to mediate the transfer of various antibiotic resistance genes. Therefore, it is advisable to use probiotics along with the feed or use them as postbiotics rather than dispersing them directly into the water in aquaculture system.

키워드

과제정보

이 연구는 국립수산과학원 (R2023058, 수산분야 항생제 통합감시 및 내성균 연구)의 지원에 의해 진행되었습니다.

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