Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Characterization of broad bean wilt virus 2 isolated from Perilla frutescens in Korea

국내 잎들깨에서 발생한 잠두위조바이러스2의 특성 구명

  • Hyun-Sun Kim (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Hee-Seong Byun (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • You-Ji Choi (Agricultural Development and Technology Center) ;
  • Hyun-Yong Choi (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Jang-Kyun Seo (Graduate School of International Agricultural Technology, Seoul National University) ;
  • Hong-Soo Choi (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Bong-Choon Lee (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Mikyeong Kim (Department of Plant Medicine, Chungbuk National University) ;
  • Hae-Ryun Kwak (Crop Protection Division, National Institute of Agricultural Sciences, Rural Development Administration)
  • 김현선 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 변희성 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 최유지 (금산군 농업기술센터) ;
  • 최현용 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 서장균 (서울대학교 국제농업기술학과) ;
  • 최홍수 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 이봉춘 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 김미경 (충북대학교 식물의학과) ;
  • 곽해련 (농촌진흥청 국립농업과학원 작물보호과)
  • Received : 2022.11.24
  • Accepted : 2023.02.14
  • Published : 2023.03.31
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Abstract

Broad bean wilt virus 2 (BBWV2) is a species in the genus Fabavirus and family Secoviridae, which is transmitted by aphids and has a wide host range. The BBWV2 genome is composed of two single-stranded, positive-sense RNAs, RNA-1 and RNA-2. The representative symptoms of BBWV2 are mosaic, mottle, vein clearing, wilt, and stunting on leaves, and these symptoms cause economic damage to various crops. In 2019, Perilla fructescens leaves with mosaic and yellowing symptoms were found in Geumsan, South Korea. Reverse-transcription polymerase chain reaction (RT-PCR) was performed with specific primers for 10 reported viruses, including BBWV2, to identify the causal virus, and the results were positive for BBWV2. To characterize a BBWV2 isolate (BBWV2-GS-PF) from symptomatic P. fructescens, genetic analysis and pathogenicity tests were performed. The complete genomic sequences of RNA-1 and RNA-2 of BBWV2-GS-PF were phylogenetically distant to the previously reported BBWV2 isolates, with relatively low nucleotide sequence similarities of 76-80%. In the pathogenicity test, unlike most BBWV2 isolates with mild mosaic or mosaic symptoms in peppers, the BBWV2-GS-PF isolate showed typical ring spot symptoms. Considering these results, the BBWV2-GS-PF isolate from P. fructescens could be classified as a new strain of BBWV2.

잠두위조바이러스2 (BBWV2)는 경제적으로 중요한 원예, 관상작물에 심각한 피해를 주는 바이러스 중의 하나다. BBWV2의 넓은 기주 범위, 매개충 방제의 어려움 등 효과적인 치료제가 없기 때문에, 병을 예방하거나 저항성 품종의 개발 등을 위해서는 BBWV2의 새로운 분리주들의 특성 조사와 연구가 필요하다. 본 연구에서는 2019년 금산지역의 잎들깨 재배농가에서 분리된 BBWV2 분리주(BBWV2-GS-PF)의 유전학적·생물학적 특성을 구명하고 기존에 보고된 분리주들과의 특성을 비교하였다. 순수 분리된 BBWV2-GS-PF는 기존 분리주와 달리 들깨에서 심한 모자이크 증상과 고추에서 원형 반점 증상을 보였다. BBWV2-GS-PF의 유전자 계통분석 결과, 기존에 알려진 2개의 그룹과 약 76~80%의 비교적 낮은 유전자 상동성을 보이며 계통이 분화된 것을 확인할 수 있었다.

Keywords

Acknowledgement

This research was supported by a grant from the Agenda Program (PJ01491302), funded by the Rural Development Administration of Korea.

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