The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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First Report of Root Rot of Dendropanax trifidus Caused by Fusarium oxysporum in Korea

Fusarium oxysporum에 의한 황칠나무 묘목 뿌리썩음병 발생 보고

  • Hyerin Ma (Department of Forest Resources, College of Agriculture and Life Sciences, Chonnam National University) ;
  • Sungyu Choi (Department of Integrative Food Bioscience and Biotechnology, Chonnam National University) ;
  • Hyunkyu Sang (Department of Integrative Food Bioscience and Biotechnology, Chonnam National University) ;
  • Hyun-Jun Kim (Department of Forest Resources, College of Agriculture and Life Sciences, Chonnam National University)
  • 마혜린 (전남대학교 농업생명과학대학 산림자원학과) ;
  • 최선규 (전남대학교 융합식품바이오공학과) ;
  • 상현규 (전남대학교 융합식품바이오공학과) ;
  • 김현준 (전남대학교 농업생명과학대학 산림자원학과)
  • Received : 2022.12.06
  • Accepted : 2023.03.28
  • Published : 2023.03.31
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Abstract

Dendropanax trifidus belonging to the family Araliaceae, is a warm-temperate evergreen tree distributed in Jeju Island, Bogil Island, Geomun Island, Geoje Island, Wando, and Haenam in Korea. In June 2021, a root rot disease in which branches of Dendropanax trifidus seedlings turned brown and shrunk was discovered at the seedling cultivation facility in Naju-si, Republic of Korea. To identify the root rot fungus, three strains were isolated from the diseased tissues of seedlings and their mycological characteristics were investigated on potato dextrose agar. In addition, a molecular phylogenetic analysis was performed using sequences of the internal transcribed spacer (ITS) region and translation elongation factor 1-α (EF1-α) gene. The fungus was identified as Fusarium oxysporum. For pathogenicity test, the roots of seedlings were immersed in the conidia suspension of the strains and planted. After 20 days inoculation, root rot and browning symptoms were confirmed in the inoculated plants. This is the first report of F. oxysporum on D. trifidus in Korea.

황칠나무는 두릅나무과에 속하는 난대성 상록활엽수로 우리나라 제주도, 보길도, 거문도, 거제도, 완도, 해남 등에 분포한다. 2021년 6월 전라남도 나주에서 황칠나무 유묘의 지제부가 갈변하고 잘록해지는 뿌리썩음병을 국내 최초로 발견하였다. 황칠나무 뿌리썩음병균을 분리 및 동정하기 위해, 유묘의 병든 조직에서 3개의 균주를 분리하였고, PDA 배지에서 형태적, 균학적 특성 조사한 결과 Fusarium oxysporum으로 동정되었다. Internal transcribed spacer (ITS) 및 translation elongation factor 1-α (EF1-α) 염기서열을 통한 분자계통학적 분석을 수행하여 한국의 황칠나무 뿌리썩음병균은 F. oxysporum임을 확인하였다. 병원성 테스트를 위해 유묘의 뿌리를 분생포자 현탁액에 침지하여 정식하였고, 접종 20일 후 처리구에서 뿌리썩음 및 지제부의 갈변 증상을 확인하였다. 본 연구는 한국의 황칠나무 유묘에서 뿌리썩음병균 F. oxysporum의 첫 보고이다.

Keywords

Acknowledgement

This study was supported by grants from the Korea Forest Service (Grant No. 2020183C10-2022-AA02) and the Rural Development and Administration, Republic of Korea (PJ01690602).

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