The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Taxonomic Study on Six Yeast Species Unlisted in the National Species List of Korea

  • Chorong Ahn (Species Diversity Research Division, National Institute of Biological Resources) ;
  • Soonok Kim (Biological Resources Utilization Division, National Institute of Biological Resources) ;
  • Changmu Kim (Species Diversity Research Division, National Institute of Biological Resources)
  • Received : 2023.01.13
  • Accepted : 2023.03.13
  • Published : 2023.03.31
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Abstract

More than five hundreds of yeast species (including 9 variants) encompassing 142 genera and 48 classes of 2 phyla exist in Korea. However, only 173 species have been cataloged in the National Species List of Korea (NSLK), the backbone reference to claim sovereign rights over biological resources, as of December 2021, due to the lack of taxonomic descriptions, although some of these species are extensively used in industry. The present pilot study investigated the taxonomy of strains belonging to the six most widely used or frequently isolated yeast species (Meyeromyma guilliermondii, Saccharomyces cerevisiae, Saccharomycopsis fibuligera, Wickerhamomyces anomalus, Candida tropicalis, and Papiliotrema flavescens) to include these species in the NSLK. Strains with diverse habitats and geographic origins were retrieved from the National Institute of Biological Resources culture collection. These strains clustered in the same clade as the type strains of the designated species according to phylogenetic analysis of the D1/D2 sequences. Moreover, we described the cell morphology and physiological characteristics of representative strains of each species. This study suggests that these six species are indigenous to Korea and can be accordingly listed in the NSLK.

Keywords

Acknowledgement

This research was supported by the National Institute of Biological Resources (NIBR202102107, NIBR202203112) under the Ministry of Environment, Republic of Korea.

References

  1. Saito K. Notizen uber einige koreanische garungsorganismen. Cent f Bakt II Abt Bd 1910;26:369-73.
  2. Ahn C, Kim M, Kim C. Comprehensive review of indigenous yeast species in Korea: a literature and culture collection analysis. Korean J Mycol 2021;49:211-42.
  3. Kurtzman CP, Robnett CJ. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek 1998;73:331-71. https://doi.org/10.1023/A:1001761008817
  4. Kumar S, Stecher G, Li M, Knyaz C, Tamura K. MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol Biol Evol 2018;35:1547-9. https://doi.org/10.1093/molbev/msy096
  5. Kurtzman CP, Fell JW, Boekhout T, Robert V. Methods for isolation, phenotypic characterization and maintenance of yeasts. In: Kurtzman CP, Fell WF, Boekhout T, editors. The yeasts, a taxonomic study, 5th edn. Amsterdam: Elsevier Science Pulishers; 2011. p. 87-110.
  6. Kurtzman CP, Suzuki M. Phylogenetic analysis of ascomycete yeasts that form coenzyme Q-9 and the proposal of the new genera Babjeviella, Meyerozyma, Millerozyma, Priceomyces, and Scheffersomyces. Mycoscience 2010;51:2-14. https://doi.org/10.1007/S10267-009-0011-5
  7. Yurkov AM, Dlauchy D, Peter G. Meyerozyma amylolytica sp. nov. from temperate deciduous trees and the transfer of five Candida species to the genus Meyerozyma. Int J Syst Evol Microbiol 2017;67:3977-81. https://doi.org/10.1099/ijsem.0.002232
  8. Kurtzman CP. Meyerozyma Kurtzman & M. Suzuki (2010). In: Kurtzman CP, Fell WF, Boekhout T, editors. The yeasts, a taxonomic study, 5th edn. Amsterdam: Elsevier Science Pulishers; 2011. p. 621-4.
  9. Naseeb S, James SA, Alsammar H, Michaels CJ, Gini B, Nueno-Palop C, Bond CJ, McGhie H, Roberts IN, Delneri D. Saccharomyces jurei sp. nov., isolation and genetic identification of a novel yeast species from Quercus robur. Int J Syst Evol Microbiol 2017;67:2046-52. https://doi.org/10.1099/ijsem.0.002013
  10. Vaughan-Martini A, Martini A. Saccharomyces Meyen ex Reess (1870). In: Kurtzman CP, Fell JW, Boekhout T, editors. The yeasts, a taxonomic study. 5th ed. Amsterdam: Elsevier; 2011. p. 733-46.
  11. Schioenning HL. Nouveau genre de la famille des Saccharomycetes. Compt Rend Trav Lab Carlsberg 1903;6:101-25.
  12. Hajihosseinali M, Nasr S, Amoozegar MA, Yurkov A. Saccharomycopsis oxydans sp. nov., a new non-fermentative member in the genus Saccharomycopsis isolated from a traditional dairy product of Iran. Int J Syst Evol Microbiol 2020;70:1059-63. https://doi.org/10.1099/ijsem.0.003874
  13. Yuan X, Peng K, Li C, Zhao Z, Zeng X, Tian F, Li Y. Complete genomic characterization and identification of Saccharomycopsis phalluae sp. nov., a novel pathogen causes yellow rot disease on Phallus rubrovolvatus. J Fungi 2021;7:707.
  14. Chi Z, Chi Z, Liu G, Wang F, Ju L, Zhang T. Saccharomycopsis fibuligera and its applications in biotechnology. Biotechnol Adv 2009; 27:423-31. https://doi.org/10.1016/j.biotechadv.2009.03.003
  15. Lee TS, Lee SK, Kim SS, Yoshida T. Microbiogical studies of red pepper paste fermentation (part I): the distribution of the yeasts during the red pepper paste fermentation periods. Korean J Mycol 1970;8:151-62.
  16. Kurtzman CP, Robnett CJ, Basehoar-Powers E. Phylogenetic relationships among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyces gen. nov. FEMS Yeast Res 2008;8:939-54. https://doi.org/10.1111/j.1567-1364.2008.00419.x
  17. Nundaeng S, Suwannarach N, Limtong S, Khuna S, Kumla J, Lumyong S. An updated global species diversity and phylogeny in the genus Wickerhamomyces with addition of two new species from Thailand. J Fungi 2021;7:957.
  18. Chang CF, Hsu IM, Liu CH, Lee CF. Wickerhamomyces sinyiensis fa, sp. nov., a new ascomycetous yeast species in the Wickerhamomyces clade isolated in Taiwan. Int J Syst Evol Microbiol 2022;72:005466.
  19. Hittinger CT, Steele JL, Ryder DS. Diverse yeasts for diverse fermented beverages and foods. Curr Opin Biotechnol 2018;49:199-206. https://doi.org/10.1016/j.copbio.2017.10.004
  20. Guinea J. Global trends in the distribution of Candida species causing candidemia. Clin Microbiol Infect 2014;20:5-10. https://doi.org/10.1111/1469-0691.12539
  21. Yan J, Jianping W, Jing B, Daoquan W, Zongding H. Phenol biodegradation by the yeast Candida tropicalis in the presence of m-cresol. Biochem Eng J 2006;29:227-34. https://doi.org/10.1016/j.bej.2005.12.002
  22. Swain MR, Krishnan C. Improved conversion of rice straw to ethanol and xylitol by combination of moderate temperature ammonia pretreatment and sequential fermentation using Candida tropicalis. Ind Crops Prod 2015;77:1039-46. https://doi.org/10.1016/j.indcrop.2015.10.013
  23. Lachance MA, Boekhout T, Scorzetti G, Fell JW, Kurtzman CP. Candida berkhout (1923). In: Kurtzman CP, Fell WF, Boekhout T, editors. The yeasts, a taxonomic study, 5th edn. Amsterdam: Elsevier Science Pulishers; 2011. p. 987-1278.
  24. Liu XZ, Wang QM, Goker M, Groenewald M, Kachalkin AV, Lumbsch HT, Millanes AM, Wedin M, Yurkov AM, Boekhout T, et al. Towards an integrated phylogenetic classification of the Tremellomycetes. Stud Mycol 2015;81:85-147. https://doi.org/10.1016/j.simyco.2015.12.001
  25. Khunnamwong P, Surussawadee J, Srisuk N, Boonmak C, Limtong S. Papiliotrema phichitensis f.a., sp. nov., a novel yeast species isolated from sugarcane leaf in Thailand. Antonie Van Leeuwenhoek 2018;111:2455-61. https://doi.org/10.1007/s10482-018-1134-5
  26. Sampaio JP. Papiliotrema Sampaio, Weiss & Bauer (2002). In: Kurtzman CP, Fell WF, Boekhout T, editors. The yeasts, a taxonomic study, 5th edn. Amsterdam: Elsevier Science Pulishers; 2011. p. 1519-21.
  27. Vancanneyt M, Coopman R, Tytgat R, Hennebert GL, Kersters K. Whole-cell protein patterns, DNA base compositions and coenzyme Q types in the yeast genus Cryptococcus Kutzing and related taxa. Syst Appl Microbiol 1994;17:65-75. https://doi.org/10.1016/S0723-2020(11)80033-0
  28. Gueho E, Improvisi L, Christen R, de Hoog GS. Phylogenetic relationships of Cryptococcus neoformans and some related basidiomycetous yeasts determined from partial large subunit rRNA sequences. Antonie van Leeuwenhoek 1993;63:175-89. https://doi.org/10.1007/BF00872392