Journal of Ginseng Research

  • 제47권3호
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  • Pages.479-491
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  • 2023
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  • 1226-8453(pISSN)
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  • 2093-4947(eISSN)
고려인삼학회 (The Korean Society of Ginseng)
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Systemic and molecular analysis dissect the red ginseng induction of apoptosis and autophagy in HCC as mediated with AMPK

  • Young Woo Kim (School of Korean Medicine, Dongguk University) ;
  • Seon Been Bak (School of Korean Medicine, Dongguk University) ;
  • Won-Yung Lee (School of Korean Medicine, Dongguk University) ;
  • Su Jin Bae (School of Korean Medicine, Dongguk University) ;
  • Eun Hye Lee (College of Medicine, Kyungpook National University) ;
  • Ju-Hye Yang (Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine) ;
  • Kwang Youn Kim (Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine) ;
  • Chang Hyun Song (MRC center, College of Korean Medicine, Daegu Haany University) ;
  • Sang Chan Kim (MRC center, College of Korean Medicine, Daegu Haany University) ;
  • Un-Jung Yun (School of Korean Medicine, Dongguk University) ;
  • Kwang Il Park (Department of Veterinary Physiology, College of Veterinary Medicine, Gyeongsang National University)
  • 투고 : 2022.10.10
  • 심사 : 2023.02.12
  • 발행 : 2023.05.01
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초록

Background: Hepatocellular carcinoma (HCC) has a high incidence and is one of the highest mortality cancers when advanced stage is proceeded. However, Anti-cancer drugs available for treatment are limited and new anti-cancer drugs and new ways to treat them are minimal. We examined that the effects and possibility of Red Ginseng (RG, Panax ginseng Meyer) as new anti-cancer drug on HCC by combining network pharmacology and molecular biology. Materials and Methods: Network pharmacological analysis was employed to investigate the systems-level mechanism of RG focusing on HCC. Cytotoxicity of RG was determined by MTT analysis, which were also stained by annexin V/PI staining for apoptosis and acridine orange for autophagy. For the analyze mechanism of RG, we extracted protein and subjected to immunoblotting for apoptosis or autophagy related proteins. Results: We constructed compound-target network of RG and identified potential pathways related to HCC. RG inhibited growth of HCC through acceleration of cytotoxicity and reduction of wound healing ability of HCC. RG also increased apoptosis and autophagy through AMPK induction. In addition, its ingredients, 20S-PPD (protopanaxadiol) and 20S-PPT (protopanaxatriol), also induced AMPK mediated apoptosis and autophagy. Conclusion: RG effectively inhibited growth of HCC cells inducing apoptosis and autophagy via ATG/AMPK in HCC cells. Overall, our study suggests possibility as new anti-cancer drug on HCC by proof for the mechanism of the anti-cancer action of RG.

키워드

과제정보

Bak SB and Lee EH would like to thank to the Ph.D.'s program of Dongguk University and Kyungpook National University for completing their thesis through this work, respectively.

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