Advances in nano research

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One-step phyto-mediated fabrication of silver nanoparticles and its anti-microbial properties

  • Velmurugan Palanivel (Centre for Research Bharath Institute of Higher Education and Research) ;
  • Sung-Chul Hong (Department of Bioenvironmental Energy, College of Natural Resource and Life Science, Pusan National University) ;
  • Veera Ravi Arumugam (Centre for Research Bharath Institute of Higher Education and Research) ;
  • Sivakumar Subpiramaniyam (Department of Bioenvironmental Energy, College of Natural Resource and Life Science, Pusan National University) ;
  • Pyong-In Yi (Department of Bioenvironmental Energy, College of Natural Resource and Life Science, Pusan National University) ;
  • Seong-Ho Jang (Department of Bioenvironmental Energy, College of Natural Resource and Life Science, Pusan National University) ;
  • Jeong-Min Suh (Department of Bioenvironmental Energy, College of Natural Resource and Life Science, Pusan National University) ;
  • Eun-Sang Jung (Department of Bioenvironmental Energy, College of Natural Resource and Life Science, Pusan National University) ;
  • Je-Sung Park (Department of Bioenvironmental Energy, College of Natural Resource and Life Science, Pusan National University)
  • Received : 2022.04.12
  • Accepted : 2023.02.02
  • Published : 2023.04.25
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Abstract

This manuscript describes the one-step eco-friendly green fabrication of silver nanoparticles (AgNPs) through the in-situ bio-reduction of an aqueous solution of silver nitrate using Syzygium aromaticum leaf extract. UV-vis spectroscopy shows a characteristic SPR peak around 442 nm. FTIR spectroscopy showed that the AgNPs were capped with bioactive phyto-molecules. TEM images revealed oval and spherical particles with a mean diameter of ~12.6 nm. XRD analysis revealed crystalline and face-cantered cubic AgNPs. The phytosynthesized AgNPs showed broad-spectrum anti-microbial activity against two foodborne pathogenic bacteria, Listeria monocytogenes and Staphylococcus aureus. The AgNPs showed a prominent ability to inhibit biofilms formed by L. monocytogenes and S. aureus in laboratory conditions through a crystal violet assay. The results suggest that the AgNPs could be a novel nanotool to develop effective antimicrobial and anti-biofilm agents in food preservation.

Keywords

Acknowledgement

This work was supported by a 2-Year Research Grant of Pusan National University.

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