Journal of Korean Medicine Rehabilitation (한방재활의학과학회지)

The society of Korean Medicine Rehabilitation (한방재활의학과학회)
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The Effects of Samul-tang-ga-dansam for Wound Healing

사물탕(四物湯) 가(加) 단참(丹參)의 상처 치료에 대한 효과

  • Eun-Byeol Lee (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Daegu Haany University) ;
  • Hyeon-Ji Kim (Department of Korean Medicine Rehabilitation, Daegu Korean Medicine Hospital, Daegu Haany University) ;
  • Chae-Young Kim (Department of Korean Medicine Rehabilitation, Daegu Korean Medicine Hospital, Daegu Haany University) ;
  • Ji-Su Choi (Department of Korean Medicine Rehabilitation, Daegu Korean Medicine Hospital, Daegu Haany University) ;
  • Chang-Hoon Woo (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Daegu Haany University) ;
  • Young-Jun Kim (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Daegu Haany University) ;
  • Hee-Duk An (Department of Korean Medicine Rehabilitation, College of Korean Medicine, Daegu Haany University)
  • 이은별 (대구한의대학교 한의과대학 한방재활의학교실) ;
  • 김현지 (대구한의대학교 부속 대구한방병원 한방재활의학과) ;
  • 김채영 (대구한의대학교 부속 대구한방병원 한방재활의학과) ;
  • 최지수 (대구한의대학교 부속 대구한방병원 한방재활의학과) ;
  • 우창훈 (대구한의대학교 한의과대학 한방재활의학교실) ;
  • 김영준 (대구한의대학교 한의과대학 한방재활의학교실) ;
  • 안희덕 (대구한의대학교 한의과대학 한방재활의학교실)
  • Received : 2023.03.15
  • Accepted : 2023.04.06
  • Published : 2023.04.30
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Abstract

Objectives The purpose of this study was to evaluate the antioxidant, anti-inflammatory and wound healing effects of Samul-tang-ga-dansam water extract (SD) in wound-induced mice. Methods The mice were divided into five groups (n=7): the normal group, the control group, the positive control group, the low-dose SD group and the high-dose SD group. The normal group had no wounds and the other groups were wounded on the back with a leather punch. Distilled water was administered to the control group, 200 mg/kg of vitamin E was administered to the positive control group. In the low-dose SD group and the high-dose SD group, 1.23 g/kg and 2.47 g/kg of SD were administered, respectively. Antioxidant and anti-inflammatory protein levels were evaluated using western blot analysis. Skin tissue was analyzed by H&E, Masson's trichrome staining method. Results Oral administration of the SD significantly reduced the visible skin damage and decreased the reactive oxygen species and ONOO- activity of the serum. It significantly increased heme oxygenase-1, superoxide dismutase, catalase, GPx-1/2, Nrf2 and Keap-1 which are antioxidant-related factors in skin tissue and reduced NF-κB p65, inducible nitric oxide synthase, cyclooxygenase-2, tumor necrosis factor α, interleukin (IL)-1β, IL-6 which are inflammation-related factors. Also, SD significantly decreased NOX2, p22phox and p47phox and increased α-smooth muscle actin and COL1A1 protein expression in fibroblasts involved in connective tissue repair. According to histological examination, the thickened epithelial layer was thinned and collagen fibers were increased to accelerate wound healing. Conclusions It is suggested that Samul-tang-ga-dansam has antioxidant and anti-inflammatory effects and promotes wound tissue repair.

Keywords

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