한국재료학회지 (Korean Journal of Materials Research)

  • 제33권4호
  • /
  • Pages.130-134
  • /
  • 2023
  • /
  • 1225-0562(pISSN)
  • /
  • 2287-7258(eISSN)
한국재료학회 (Materials Research Society of Korea)
권호 표지
DOI QR코드

DOI QR Code

Cytotoxicity of Impregnated Dental Gingival Retraction Cord Extracts in Immortalized Human Oral Fibroblasts and Keratinocytes in vitro

  • Myung-Jin Lee (Department of Dental Hygiene, Division of Health Science, Baekseok University) ;
  • Song-Yi Yang (Department of Dental Hygiene, Konyang University)
  • 투고 : 2023.02.21
  • 심사 : 2023.04.04
  • 발행 : 2023.04.27
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

This study evaluated cell viability and cytokine release in immortalized human oral fibroblasts (hTERT-hNOFs) and keratinocytes (IHOK) exposed to a dental-impregnated gingival retraction cord. To prepare the extracts, dental gingival retraction cords impregnated with aluminum chloride hexahydrate were immersed in a cell culture medium for 24 h at 37 ℃. hTERT-hNOFs and IHOK were cultured for 24 h. The cell culture medium was removed and extracts of the dental gingival retraction cords were added. After incubation with the extract solution, cell viability was evaluated using an MTT assay. The levels of the cytokines IL-1α and IL-8 were measured in the supernatants of each cell type. The cell viability after exposure to the extract solution for 10 min exceeded 70 % in both cell types. The ET50 values for hTERT-hNOF and IHOK were 35.75 and 28.98 min, respectively. For IHOK, the IL-1α level was (5.35 ± 5.22) pg/mL at 10 min, (3.58 ± 5.38) pg/mL at 20 min, and (2.85 ± 4.28) pg/mL at 60 min of exposure (p > 0.05). The IL-8 level in IHOK was (67.16 ± 18.70) pg/mL at 10 min, (78.36 ± 7.50) pg/mL at 20 min, and (111.9 ± 26.10) pg/mL at 60 min of exposure (p > 0.05). Cytokine release was not observed from hTERT-hNOFs. Based on these results, cell viability and cytokine release were confirmed in cells exposed to the impregnated gingival retraction cord. In addition, the application of the extracts to hTERT-hNOF and IHOK during the actual contact time and determination of ET50 may be beneficial for evaluating the biocompatibility of dental-impregnated gingival retraction cords.

키워드

과제정보

Funding for this project was through the Ministry of Education of the Republic of Korea and the National Research Foundation of Korea (NRF-2020R1I1A1A01051962).

참고문헌

  1. R. G. Craig and Z. Sun, Oper. Dent., 19, 138 (1994).
  2. P. Aimjirakul, T. Masuda, H. Takahashi and H. Miura, Int. J. Prosthodont., 16, 385 (2003).
  3. T. E. Donovan and W. W. Chee, Dent. Clin. North Am., 48, 433 (2004). https://doi.org/10.1016/j.cden.2003.12.012
  4. W. W. L. Chee and T. E. Donovan, J. Prosthet. Dent., 68, 728 (1992).
  5. N. Perakis, U. C. Belser and P. Magne, Int. J. Periodontics Restorative Dent., 24, 109 (2004).
  6. K. J. Shrivastava, A. Bhoyar, S. Agarwal, S. Shrivastava, S. Parlani and V. Murthy, J. Nat. Sci. Biol. Med., 6, S53 (2015).
  7. A. Jokstad, J. Prosthet. Dent., 81, 258 (1999).
  8. S. Dogan, E. R. Schwedhelm, H. Heindl, L. Mancl and A. J Raigrodski, J. Prosthet. Dent., 114, 217 (2015).
  9. J. Ruel, P. J. Schuessler and K. Malament, J. Prosthet. Dent., 44, 508 (1980).
  10. G. G. de Gennaro, H. M. Landesman, J. E. Calhoun and J. T. Martinoff, J. Prosthet. Dent., 47, 384 (1982).
  11. I. Kopac, U. Batista, E. Cvetko and L. Marion, J. Oral Rehabil., 29, 98 (2002).
  12. T. E. Donovan, B. K. Gandara and H. Nemetz, J. Prosthet. Dent., 53, 525 (1985).
  13. International Organization for Standardization, Biological evaluation of medical devices - Part 12: Sample preparation and reference materials, ISO 10993-12, Switzerland, 2012.
  14. S. L. Wilson, M. Ahearne and A. Hopkinson, Toxicology, 327, 32 (2015).
  15. J. S. Kwon, Y. Z. Piao, S. A. Cho, S. Y. Yang, J. Kim, S. An and K. M. Kim, Materials, 8, 5372 (2015).
  16. R. P. Illeperuma, Y. J. Park, J. M. Kim, J. Y. Bae, Z. M. Che, H. K. Son, M. R. Han, K. M. Kim and J. Kim, J. Mater. Sci.: Mater. Med., 23, 753 (2012).
  17. H. J. Lee, H. Y. Guo, S. K. Lee, B. H. Jeon, C. D. Jun, S. K. Lee, M. H. Park and E. C. Kim, J. Oral Pathol. Med., 34, 436 (2005).
  18. J. H. Lee, S. H. Seo, S. B. Lee, J. Y. Om, K. M. Kim and K. N. Kim, Dent. Mater., 31, 123 (2015).
  19. A. Padbury, Jr., R. Eber and H. L. Wang, J. Clin. Periodontol., 30, 379 (2003).
  20. D. J. Weir and B. H. Williams, J. Prosthet. Dent., 51, 326 (1984).
  21. M. F. Land, S. F. Rosenstiel and J. L. Sandrik, J. Prosthet. Dent., 72, 4 (1994).
  22. C. M. Liu, F. M. Huang, L. C. Yang, L. S. Chou, M. Y. Chou and Y. C. Chang, J. Oral Rehabil., 31, 368 (2004).
  23. P. Tarighi and M. Khoroushi, Dent. Res. J., 11, 423 (2014).
  24. A. R. Buttendorf, C. F. Ferreira, M. A. Bianchini, J. E. da Silva, Russel and A. Wicks, J. Dent. Health Oral Disord. Ther., 3, 244 (2015).
  25. Q. Jiang, Y. Yu, H. Ruan, Y. Luo and X. Guo, BMC Oral Health, 14, 1 (2014).
  26. P. D. Bowman, X. Wang, M. A. Meledeo, M. A. Dubick and B. S. Kheirabadi, J. Trauma, 71, 727 (2011).
  27. K. Moharamzadeh, R. Van Noort, I. M. Brook and A. M. Scutt, Dent. Mater., 23, 40 (2007).
  28. M. Tichy, R. Valigurova, R. Cabala, R. Uzlova and M. Rucki, Interdiscip. Toxicol., 3, 73 (2010).
  29. A. Sfakianakis, C. E. Barr and D. L. Kreutzer, J. Periodontal Res., 37, 154 (2002).
  30. Q. Xu, K. Izumi, T. Tobita, Y. Nakanishi and S. E. Feinberg, J. Oral Maxillofac. Surg., 67, 1256 (2009).
  31. S. Kezic, G. M. O'Regan, R. Lutter, I. Jakasa, E. S. Koster, S. Saunders, P. Caspers, P. M. J. H. Kemperman, G. J. Puppels, A. Sandilands, H. Chen, L. E. Campbell, K. Kroboth, R. Watson, P. G. Fallon, W. H. I. McLean and A. D. Irvine, J. Allergy Clin. Immunol., 129, 1031 (2012).
  32. J. Breger, L. Baeva, A. Agrawal, E. Shindell and D. E. Godar, Photochem. Photobiol., 89, 665 (2013).
  33. T. Sakai, Y. Hatano, W. Zhang, S. Fujiwara and R. Nishiyori, J. Dermatol. Sci., 80, 158 (2015).