한국자기공명학회논문지 (Journal of the Korean Magnetic Resonance Society)

한국자기공명학회 (Korean Magnetic Resonance Society)
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H-1, C-13, and N-15 resonance assignments of ENOD40B, a plant peptide hormone

  • 투고 : 2023.05.16
  • 심사 : 2023.06.10
  • 발행 : 2023.06.20
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초록

t ENOD40B, a plant peptide hormone, was doubly labeled with C-13 and N-15 by recombinant production in Escherichia coli. The peptide was prepared by affinity chromatography followed by protease cleavage and reverse-phase chromatography. To elucidate the mode of action against its receptor, sucrose synthase, we proceeded to assign the backbone and side-chain resonances using a set of double and triple resonance experiments. This result will be used to determine the three-dimensional structure of the peptide at its bound state as well as to observe the chemical shift changes upon binding.

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과제정보

This work was supported by the National Research Foundation of Korea (NRF-2021R1F1A1046500), and the faculty research fund of Sejong University in 2023. The author thanks Dr. Marco Tonelli of the NMRFAM for his technical assistance.

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