Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Evaluation of subchronic oral dose toxicity and allergen of freeze-dried powder of Locusta migratoria (Orthoptera: Acrididae) as a novel food source

  • Sun Young Kim (Industrial Insect Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kyu‑Won Kwak (Industrial Insect Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Ji Yeong Park (Industrial Insect Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Eun‑Sung Park (Headquarters of GLP, Nonclinical Research Institute, Chemon Inc.) ;
  • Chun‑Ja Nam (Headquarters of GLP, Nonclinical Research Institute, Chemon Inc.) ;
  • Kyu Sup An (Bioconvergence Technology Laboratory, Korea Conformity Laboratories) ;
  • Hyun‑Jin Kim (Jeon-Nam Agricultural Research & Extension Services, Insect & Sericultural Research Institute) ;
  • Hyung Joo Yoon (Industrial Insect Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Yong‑Soon Kim (Industrial Insect Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kwanho Park (Industrial Insect Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Eunsun Kim (Industrial Insect Division, Department of Agricultural Biology, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Hyeon Yeol Ryu (Bioconvergence Technology Laboratory, Korea Conformity Laboratories) ;
  • Sun‑Don Kim (Headquarters of GLP, Nonclinical Research Institute, Chemon Inc.)
  • Received : 2022.10.12
  • Accepted : 2023.02.01
  • Published : 2023.04.15
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Abstract

The migratory locust, Locusta migratoria (Orthoptera: Acrididae), is a well-known edible insect which may serve as new source of human food and animal feed. However, potential toxicity and food safety of L. migratoria had not been investigated extensively until now. Therefore, in this study, we aimed to investigate toxicity of freeze-dried powder of L. migratoria (fdLM) and identify allergic components in ELISA and PCR techniques. In this subchronic study, fdLM was administered once daily by oral gavage at the doses of 750, 1500, and 3000 mg/kg/day. No toxicological changes were observed in both sexes of rats for 13 weeks in accordance with the OECD guidelines and GLP conditions. In addition, fdLM did not induced increases of serum immunoglobulin E and 21 homologous proteins were not detected under our present conditions. In conclusion, the NOAEL (no-observed-adverse-efect level) was 3000 mg/kg/day and no target organ was identifed in both sexes. In conclusion, we found that fdLM is safe with no adverse efects and ofers the potential of its use as an edible ingredient or other biological uses.

Keywords

References

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