Korean Journal of Environmental Agriculture (한국환경농학회지)

The Korean Society of Environmental Agriculture (한국환경농학회)
권호 표지
DOI QR코드

DOI QR Code

Effects of Acclimatization to Different Light Colors on the Growth of Petunia (Petunia hybrida) in a Greenhouse

조직배양 페튜니아의 순화과정에서 광질에 따른 생장반응 특성

  • Young-Sun Kim (Department of Horticultural Science, Daegu University) ;
  • Geung-Joo Lee (Department of Horticulture and Department of Smart Agriculture Systems, Chungnam National University)
  • 김영선 (대구대학교 원예학과) ;
  • 이긍주 (충남대학교 원예학과/스마트농업학과)
  • Received : 2023.03.07
  • Accepted : 2023.03.23
  • Published : 2023.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

Light is an important factor that influences the growth and development of flowering plants. The present study investigated the effects of in vitro acclimatization to different light colors (white light (WL; control), blue light (BL; 447 nm), green light (GL; 519 nm), and red light (RL; 667 nm)) on the growth of petunia (Petunia hybrida) and of hardening cultivation of plant transferred form in vitro to a greenhouse under sunlight. Compared to the control, the shoot length and leaf width of Petunia increased by 42% and 11.7%, respectively, after acclimatization to BL and the shoot growth increased by 29.3% after acclimatization to RL. The chlorophyll and carotenoid contents after acclimatization to BL and GL were 16.7% and 11.3% higher, respectively, and 14.4% and 11.9% higher, respectively, than those in the control. During greenhouse cultivation, the shoot length increased by 16.7% and 11.3%, respectively, after acclimatization to BL and RL, respectively, and the leaf length and leaf width increased by 14.4% and 11.9%, respectively, after acclimatization to GL. While dry weight of root of GL and BL was not significant difference in vitro, increased by 59.0% and 22.9% ex vitro than that of WL. Thus, acclimatization to BL increased the shoot growth and leaf chlorophyll contents, and acclimatization to GL and RL enhanced shoot and root growth, in petunia.

Keywords

Acknowledgement

This research was funded by the New Breeding Technologies Development Program (No. PJ016547), Rural Development Administration.

References

  1. Song CY, Bang CS (2001) Correlation and combining ability of plant height and characters relared to flowering of F1 hybrids by dialle cross in Petunia hybrida. Journal of the Korean Society for Horticultural Science, 42(5), 601-605.
  2. Lee JK, Park JY, Park KJ, Ryu SH, Shin JH (2004) Genetic studies of major agronomic traits in hybrid populations of maize inbred lines. Korean Journal of Plant Resources, 17(3), 304-313.
  3. Moon HK, Park SY (2008) Effect of different light sources and ventilation on in vitro shoot growth and rooting of a rare and endangered species, Tsuru-rindo (Tripterospermum japonicum). Journal of Plant Biotechnology, 35(3), 215-221. https://doi.org/10.5010/JPB.2008.35.3.215
  4. Kim JB (2017) Optimization of a protocol for the production of transgenic lily plants via particle bombardment. Journal of Plant Biotechnology, 44(1), 82-88. https://doi.org/10.5010/JPB.2017.44.1.082.
  5. Li CH, Lim JD, Kim MJ, Kim NY, Yu CY (2005) Acclimatization and growth characteristics of plantlets of Eleutherococcus senticosus maxim cultured by bioreactor. Journal of Medicinal Crop Science, 13(4), 133-137.
  6. Kwon SI, Kim, MJ, Kang IK (2005) Changes of root physiology of tissue cultured M.9 apple rootstock after layering. Korean Journal of Plant Biotechnology, 32, 181-186. https://doi.org/10.5010/JPB.2005.32.3.181
  7. Shin YK, Yoon YTJ, Hahn EJ, Paek KY (2009) Photosynthetic photon flux (PPF) affects growth, photosynthesis and acclimatization of Phalaenosis 'Amaglde' plantlets. Korean Journal of Horticultural Science and Technology, 27, 476-481.
  8. Heo JW, Lee JS, Lee GI, Kim HH (2017) Growth of kale seedling affected by the control of light of quality and intensity under smart greenhouse conditions with artificial lights. Korean Journal of Environmental Agriculture, 36, 193-200. https://doi.org/10.5338/KJEA.2017.36.3.31.
  9. Kim S, Kim J, Oh W (2018) Propagation efficiencies at different LED light qualities for leaf cutting of six echeveria cultivars in a plant factory system. Protected Horticulture and Plant Factory, 27(4), 363-370. https://doi.org/10.12791/KSBEC.2018.27.4.363.
  10. Zheng L, Steppe K, Van Labeke MC (2020) Spectral quality of monochromatic LED affects photosynthetic acclimation to high-intensity sunlight of Chrysanthemum and Spathiphyllum. Physiologia Plantarum, 169(1), 10-26. https://doi.org/10.1111/ppl.13067.
  11. Kim S, Bok G, Park J (2018) Analysis of antioxidant content and growth of Agastache rugosa as affected by LED light qualities. Journal of Bio-Environment Control, 27, 260-268. https://doi.org/10.12791/KSBEC.2018.27.3.260.
  12. Yoo KR, Lee SY (2017) Effects of light-emitting diodes on in vitro growth of virus-free sweet potato plantlets. Korean Journal of Horticultural Science and Technology, 35(4), 490-498. https://doi.org/10.12972/kjhst.20170052.
  13. Lichtenthaler HK, Buschmann C (2001) Chlorophylls and carotenoids: Measurement and characterization by UV-VIS spectroscopy. Current Protocols in Food Analytical Chemistry, 1(1), F4.3.1-F4.3.8.
  14. Heo JW, Kim HH, Yoon JB, Lee JK, Huh YS, Lee KY (2015) Effect of supplementary radiation on growth of greenhouse-grown kales. Korean Journal of Environmental Agriculture, 34, 38-45. https://doi.org/10.5338/KJEA.2015.34.1.02.
  15. Heo JW, Lee YB, Chang YS, Lee JT, Lee DB (2010) Effects of light quality and lighting type using an LED chamber system on chrysanthemum growth and development cultured in vitro. Korean Journal of Environmental Agriculture, 29, 374-380. https://doi.org/10.5338/KJEA.2010.29.4.374.
  16. Kim YH, Lee JS. (2016) Growth and contents of anthocyanins and ascorbic acid in lettuce as affected by supplemental UV-A LED irradiation with different light quality and photoperiod. Korean Journal of Horticultural Science and Technology, 34, 596-606. https://doi.org/10.12972/kjhst.20160061.
  17. Lin KH, Huang MY, Huang WD, Hsu MH, Yang ZW, Yang CM (2013) The effects of red, blue and white light-emitting diodes on the growth development, and edible quality of hydrophonically grown lettuce (Lactuca sativa L. var. capitata). Sientia Horticulturae, 150, 86-91. https://doi.org/10.1016/j.scienta.2012.10.002.
  18. Lee JH, Nam SY (2022) Analysis of growth and leaf color changes of Sedum album cv. Athoum according to the sepectral power distribution of several white LEDs. Flower Research Journal, 30(4), 184-193. https://doi.org/10.11623/frj.2022.30.4.03.
  19. Heo JW, Lee CW, Chakrabarty D, Paek KY (2002) Growth responses of marigold and salvia bedding plants as affected by monochromic or mixture radiation provided by a light-emitting diode (LED). Plant Growth Regulation, 38, 225-230. https://doi.org/10.1023/A:1021523832488.
  20. Zuo Z, Liu H, Liu B, Liu X, Lin, C (2011) Blue lightdependent interaction of CRY2 with SPA1 regulates COP1 activity and floral initiation in Arabidopsis. Current Biology, 21(10), 841-847. https://doi.org/10.1016/j.cub.2011.03.048.
  21. Xue H, Che X, Li G (2007) Involvement of phospholipid signaling in plant growth and hormone effects. Current Opinion in Plant Biology, 10, 483-489. https://doi.org/10.1016/j.pbi.2007.07.003.
  22. Maluta FA, Bordignon SR, Rossi ML, Ambrosano GMB, Rodrigues PHV (2013) In vitro culture of sugarcane exposed to different light sources. Pesquisa Agropecuaria Brasileira, 48, 1303-1307. https://doi.org/10.1590/S0100-204X2013000900015.
  23. Hung CD, Hong CH, Kim SK, Lee KH, Park JY, Dung CD, Nam MW, Choi DH, Lee HI (2016) In vitro proliferation and ex vitro rooting of microshoots of commercially important rabbiteye blueberry (Vaccinium ashei Reade) using spectral lights. Scientia Horticulurae, 211, 248-254. https://doi.org/10.1016/j.scienta.2016.09.003.
  24. Miazek K, Ledakowicz S (2013) Chlorophyll extraction from leaves, needles and microalgae: A kinetic approach. International Journal of Agricultural and Biological Engineering, 6, 107-115. http://doi.org/10.3965/j.ijabe.20130602.0012.
  25. Lee JJ, Kim YS, Ham SK, Lee CE, Lee GJ. (2015) Growth and quality improvement of creeping bentgrass by two fertilizer containing Trichoderma species. Weed and Turfgrass Science, 4(3), 249-255. https://doi.org/10.5660/WTS.2015.4.3.249.
  26. Kim YS, Lee TS, Cho SH, Lee GJ (2018) Application of liquid fertilizer containing humate improving rhizosphere activation and favoring turfgrass quality. Weed and Turfgrass Science, 7(1), 62-71. https://doi.org/10.5660/WTS.2018.7.1.62.
  27. Kim YS, Lee TS, Cho SH, Jeong JY, An JY, Lee JJ, Han KP, Hong JH (2017) Growth effect of mixed organic fertilizer blending poultry manure compost in leaf vegetables. Journal of the Korea Organic Resources Recycling Association, 25(3), 45-54. https://doi.org/10.17137/korrae.2017.25.3.45.
  28. Arias JP, Zapata K, Rojano B, Arias M (2016) Effect of light wavelength on cell growth, content of phenolic compounds and antioxidant activity in cell suspension cultures of Thevetia peruviana. Journal of Photochemistry and Photobiology B: Biology, 163, 87-91. https://doi.org/10.1016/j.jphotobiol.2016.08.014.
  29. Daud N, Faizal A, Geelen D (2013) Adventitious rooting of Jatropha curcas L. is stimulated by phloroglucinol and by red LED light. In Vitro Cellular & Developmental Biology-Plant, 49, 183-190. https://doi.org/10.1007/s11627-012-9486-4.
  30. Folta KM, Maruhnich SA (2007) Green light: a signal to slow down or stop. Journal of Experimental Botany, 58, 3099-3111. https://doi.org/10.1093/jxb/erm130.