Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Protection of Skin Fibroblasts from Infrared-A-Induced Photo-Damage Using Ginsenoside Rg3(S)-Incorporated Soybean Lecithin Liposomes

  • Won Ho Jung (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Jihyeon Song (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Gayeon You (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Jun Hyuk Lee (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Sin Won Lee (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Joong-Hoon Ahn (Department of Bioscience and Biotechnology, Konkuk University) ;
  • Hyejung Mok (Department of Bioscience and Biotechnology, Konkuk University)
  • Received : 2022.10.27
  • Accepted : 2022.12.20
  • Published : 2023.01.28
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Abstract

Protection of skin cells from chronic infrared-A (IRA) irradiation is crucial for anti-photoaging of the skin. In this study, we investigated the protective activity of Rg3(S) and Rg3(S)-incorporated anionic soybean lecithin liposomes (Rg3/Lipo) with a size of approximately 150 nm against IRA-induced photodamage in human fibroblasts. The formulated Rg3/Lipo showed increased solubility in aqueous solution up to a concentration of 200 ㎍/ml, compared to free Rg3(S). In addition, Rg3/Lipo exhibited superior colloidal stability in aqueous solutions and biocompatibility for normal human dermal fibroblasts (NHDFs). After repeated IRA irradiation on NHDFs, elevated levels of cellular and mitochondrial reactive oxygen species (ROS) were greatly reduced by Rg3(S) and Rg3/Lipo. In addition, cells treated with Rg3/Lipo exhibited noticeably reduced apoptotic signals following IRA irradiation compared to untreated cells. Thus, considering aqueous solubility and cellular responses, Rg3/Lipo could serve as a promising infrared protector for healthy aging of skin cells.

Keywords

Acknowledgement

This study was supported by the Korean Society of Ginseng (2021) and the Ministry of Health and Welfare, Republic of Korea (grant no. HI22C0537).

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