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The Human Milk Oligosaccharide 2'-Fucosyllactose Shows an Immune-Enhancing Effect in a Cyclophosphamide-Induced Mouse Model

  • Seon Ha Jo (Department of Nano Bio Engineering, Seoul National University of Science and Technology) ;
  • Kyeong Jin Kim (Department of Nano Bio Engineering, Seoul National University of Science and Technology) ;
  • Soo-yeon Park (Department of Food Science and Technology, Seoul National University of Science and Technology) ;
  • Hyun-Dong Paik (Department of Food Science and Biotechnology of Animal Resources, Konkuk University) ;
  • Ji Yeon Kim (Department of Nano Bio Engineering, Seoul National University of Science and Technology)
  • 투고 : 2022.11.25
  • 심사 : 2022.12.06
  • 발행 : 2023.03.28

초록

The 2'-fucosyllactose (2'-FL) is the richest components in a human milk oligosaccharide. Several studies have reported that 2'-FL has beneficial effects in infants. However, there are few studies on its immune-enhancing effects. This research aimed to examine the immune-enhancing effect of 2'-FL on immunosuppression by cyclophosphamide (CCP) in ICR mice. Mice were orally administered distilled water or 0.5 mg/kg B.W. 2'-FL for 14 days. An immunocompromised mouse model was induced using CCP 80 mg/kg B.W. at 12-14 days. Using the CCP had effects on reducing their body weight, organ weight, spleen index, natural killer (NK) cell activity, and cytokines concentration and expression. This study also used concanavalin A-mediated T-cell proliferation to verify the immune-enhancing effects in the sample. Body weight, spleen index, organ weight, and cytokine levels were measured to estimate the immune-enhancing effects. The body weight at 14 days tended to increase, and the spleen weight and index significantly increased in the 2'-FL group compared to the CCP group. The NK cell activity increased in the 2'-FL group compared to the CCP group, but there was no significant difference. The concentration of interleukin (IL)-2 tended to recover in the 2'-FL group compared to the CCP group. The 2'-FL group showed a significant increase of IL-10 and IFN-gamma concentration compared to the CCP group. In addition, there was a trend of increased IL-10 mRNA expression compared to the CCP group. These results revealed that 2'-FL improved CCP-induced immunosuppression, suggesting that 2'-FL may have the potential to enhance the immune system.

키워드

과제정보

This work was supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, and Forestry (IPET) through the Innovational Food Technology Development Program (#119009-3), funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA).

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