Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Overexpression of S-Adenosylmethionine Synthetase in Recombinant Chlamydomonas for Enhanced Lipid Production

  • Jeong Hyeon Kim (Department of Biotechnology and Bioengineering, Chonnam National University) ;
  • Joon Woo Ahn (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Eun-Jeong Park (Aquatic Plant Variety Center, National Institute of Fisheries Science) ;
  • Jong-il Choi (Department of Biotechnology and Bioengineering, Chonnam National University)
  • Received : 2022.12.07
  • Accepted : 2023.01.09
  • Published : 2023.03.28
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Abstract

Microalgae are attracting much attention as promising, eco-friendly producers of bioenergy due to their fast growth, absorption of carbon dioxide from the atmosphere, and production capacity in wastewater and salt water. However, microalgae can only accumulate large quantities of lipid in abiotic stress, which reduces productivity by decreasing cell growth. In this study, the strategy was investigated to increase cell viability and lipid production by overexpressing S-adenosylmethionine (SAM) synthetase (SAMS) in the microalga Chlamydomonas reinhardtii. SAM is a substance that plays an important role in various intracellular biochemical reactions, such as cell proliferation and stress response, and the overexpression of SAMS could allow cells to ithstand the abiotic stress and increase productivity. Compared to wild-type C. reinhardtii, recombinant cells overexpressing SAMS grew 1.56-fold faster and produced 1.51-fold more lipids in a nitrogen-depleted medium. Furthermore, under saline-stress conditions, the survival rate and lipid accumulation were 1.56 and 2.04 times higher in the SAMS-overexpressing strain, respectively. These results suggest that the overexpression of SAMS in recombinant C. reinhardtii has high potential in the industrial-scale production of biofuels and various other high-value-added materials.

Keywords

Acknowledgement

This work was supported by a National Research Foundation of Korea (NRF) and Korea Foundation for Women In Science, Engineering and Technology (WISET) (WISET-2022-083) grant funded by the Korean government (MSIT) (NRF-2018R1D1A1B07049359), and the research program of the Korea Atomic Energy Research Institute (KAERI).

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