Journal of Environmental Science International (한국환경과학회지)

The Korean Environmental Sciences Society (한국환경과학회)
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Culture Conditions for Improving Manipulation Efficiency of Rat Embryo

랫드 배아 조작 효율 향상을 위한 배양 조건

  • Ji Min Lee (Department of Laboratory Animal Medicine, College of Veterinary Medicine, Seoul National University)
  • 이지민 (서울대학교 수의과대학 실험동물의학교실)
  • Received : 2023.03.12
  • Accepted : 2023.03.21
  • Published : 2023.03.31
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Abstract

Rats are one of the most widely used animals in biomedical sciences because their metabolism and physiology are comparable to humans. In recent years, gene-targeted models have been developed using various animal species utilizing engineered nucleases such as clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated gene (Cas). It has recently become possible to efficiently transfect CRISPR/Cas into embryos via electroporation. However, electroporation can damage fertilized eggs; therefore, it is important to determine the optimal embryo culture conditions. A standardized approach for routine and reproducible rat transgenesis will render rat models more accessible for research. We performed experiments to obtain rat embryos with efficient superovulation and synchronization, and to investigate the appropriate medium conditions for pronuclear stage embryos subjected to electroporation stimulation for the introduction of engineered nuclease.

Keywords

Acknowledgement

본 연구는 한국연구재단 연구사업(과제명: 유전자가위기술을 활용한 랫드 형질전환 모델 기반의 전립선암 발병 기전 분석, 과제번호: NRF- 2016R1D1A1A02937331)의 지원에 의해 이루어진 것임.

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