Journal of the Korean Magnetic Resonance Society (한국자기공명학회논문지)

Korean Magnetic Resonance Society (한국자기공명학회)
권호 표지
DOI QR코드

DOI QR Code

Pressure titration of the monomeric variant of transthyretin

  • Bokyung Kim (Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology) ;
  • Jin Hae Kim (Department of New Biology, Daegu Gyeongbuk Institute of Science and Technology)
  • Received : 2023.03.17
  • Accepted : 2023.03.20
  • Published : 2023.03.20
Download PDF
⟨ Previous Next ⟩

Abstract

Transthyretin (TTR) is an indispensable transporter protein of thyroxine and a retinol molecule in humans. TTR has a stable homo-tetrameric structure in its native state, while upon dissociation into monomers, it becomes aggregation-prone and can form an amyloid fibril. Although the amyloidogenic propensity of TTR has been known and investigated since the late 1990s, the structural information regarding TTR's amyloidogenic species is still elusive. Here, we employed high-pressure nuclear magnetic resonance (HP-NMR) approaches on the monomeric variant of TTR (TTR[F87M/L110M]; M-TTR) and observed that it experiences a two-step transition in response to the pressurized condition. Our study demonstrated that M-TTR in an ambient condition has heterogeneous structural features, which is likely related to the amyloidogenic propensity of TTR.

Keywords

Acknowledgement

We are indebted to Dr. Markus Zweckstetter for his critical help to envision the HP-NMR experiments for M-TTR. This research was supported by the National Research Foundation (NRF) funded by the Ministry of Science & ICT (NRF-2018R1C1B6008282).

References

  1. J. M. Griffin, J. L. Rosenthal, J. L. Grodin, M. S. Maurer, M. Grogan, and R. K. Cheng, JACC Cardio. Oncology 3, 488 (2021) 
  2. A. Wojtczak, V. Cody, J. R. Luft, and W. Pangborn, Acta Crystallogr. Sect. D Biol. Crystallogr. 52, 758 (1996) 
  3. S. M. Johnson, S. Connelly, C. Fearns, E. T. Powers, and J. W. Kelly, J. Mol. Biol. 421, 185 (2012) 
  4. M. Schmidt et al., Nat. Commun. 10, 1 (2019) 
  5. I. Iakovleva, M. Hall, M. Oelker, L. Sandblad, I. Anan, and A. E. Sauer-Eriksson, Nat. Commun. 12, 7141 (2021) 
  6. J. Oroz, J. H. Kim, B. J. Chang, and M. Zweckstetter, Nat. Struct. Mol. Biol. 24, 407 (2017) 
  7. L. M. Nguyen and J. Roche, J. Magn. Reson. 277, 179 (2017) 
  8. D. Foguel et al., Proc. Natl. Acad. Sci. U. S. A. 100, 9831 (2003) 
  9. J. H. Kim, J. Oroz, and M. Zweckstetter, Angew. Chemie - Int. Ed. 55, 16168 (2016) 
  10. B. Kim and J. H. Kim, J. Kor. Magn. Reson. Soc. 25, 8 (2021) 
  11. W. Lee, M. Rahimi, Y. Lee, and A. Chiu, Bioinformatics 37, 3041 (2021) 
  12. S. Conti et al., Biochemistry 53, 4381 (2014) 
  13. M. Groenning, R. I. Campos, D. Hirschberg, P. Hammarstrom, and B. Vestergaard, Sci. Rep. 5, 1 (2015) 
  14. W. Yang, B. S. Kim, S. Muniyappan, Y. Lee, J. H. Kim, and W. Yu, Front. Mol. Biosci. 8, 1 (2021)