Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Studies on Growth Characteristics and Propagation Method of Introduced Hop (Humulus lupulus L.) Cultivars

홉(Humulus lupulus L.) 도입 품종의 생육특성 및 영양번식 연구

  • Tae Hyun Ha (Department of Horticulture, Kongju National University) ;
  • Jae Il Lyu (Research Center of Crop Breeding for Omics and Artificial Intelligence, Kongju National University) ;
  • Jun-Hyung Lee (Department of Horticulture, Kongju National University) ;
  • Jaihyunk Ryu (Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Sang Hoon Park (Hop&Hope Agricultural Co., Ltd.) ;
  • Si-Yong Kang (Department of Horticulture, Kongju National University)
  • 하태현 (공주대학교 산업과학대학 원예학과) ;
  • 유재일 (공주대학교 작물오믹스지능육종연구센터) ;
  • 이준형 (공주대학교 산업과학대학 원예학과) ;
  • 류재혁 (한국원자력연구원 첨단방사선연구소 방사선육종연구실) ;
  • 박상훈 (홉앤호프(주)) ;
  • 강시용 (공주대학교 산업과학대학 원예학과)
  • Received : 2023.01.30
  • Accepted : 2023.02.23
  • Published : 2023.04.01
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Abstract

Domestic hop (Humulus lupulus L.) production has been suspended since the early 1990s due to foreign imports, but interest in local production is rising due to the recent craft beer boom in Korea. This study was conducted focusing on the development of growth characteristics and propagation technology for 6 introduced hop cultivars as a basic study for domestic hop production and breeding program. In the hop growth survey conducted in 2021 and 2022, the 5-year-old plants after planting generally showed a tendency to increase the height of strobile setting, strobile size, number and weight of strobile per hill compared to the 4-year-old plants. As a result of the experiment with hop vine cuttings, the average rooting rate of all cultivars was as high as 88% even in only water treatment that were not added with Atonik (Atonik, Arysta, Japan), a rooting agent. There were differences between cultivars in rooting length and rooting rate according to the Atonik treatment method. When checking the survival rate of the rooted cuttings seedlings after transplanting into the soil, it was confirmed that the survival rate of the cuttings in the tissue culture room was significantly lower than that of the cuttings in the greenhouse. However, in transplanting step, cutting plants from culture room condition was strongly inhibited plant growth because of changing environment conditions. As a results of tissue culture, the thidiazuron (TDZ) 1 ㎎/L treatment in the media generated 6 to 9 shoots/explant, while the 6-benzylaminopurine (BAP) 1 ㎎/L treatment generated only 1 to 2 shoots/explant. Therefore, it is more effective to culture by adding TDZ rather than BAP. These results indicated that the development of technology to manage stably after transplanting of cutting or micropropagating plants into potting soil is important for mass propagation of hops.

국내 홉 생산은 외국산 수입으로 1990년대 초반 이후 중단된 상태이나, 최근 수제맥주 붐의 영향으로 국내 생산에 대한 관심이 고조되고 있다. 본 연구는 국내 홉 생산 및 육종을 위한 기반연구로 외국산 도입 홉 6개 품종을 대상으로 생육특성 및 증식기술 개발중심으로 수행하였다. 2021년과 2022년 진행한 홉 생육조사에서 재식 후 4년차보다 5년차에서 일반적으로 홉의 착화고, 착화수, 구화의 크기와 무게가 증가하는 경향을 나타냈다. 홉 삽목 실험의 결과, 일반적으로 발근제 '아토닉' 처리를 하지 않은 물꽂이에서도 전체 평균 발근율이 88%로 높았지만 아토닉을 침지하거나 희석하여 관주할 경우 발근율이 93%로 증가하는 경향을 나타냈다. 아토닉 처리 방법에 따른 발근력과 발근율에는 품종간 차이가 있었다. 발근 삽목묘를 상토 이식 후 생존율을 확인 하였을 때, 조직배양실에서 삽목한 것이 온실에서 삽목한 것에 비해 상당히 생존율이 떨어졌다. 따라서 홉 삽목시에는 물꽂이 만으로도 높은 삽목 성공률을 얻을 수 있으며, 상토 이식 후 식물체를 안정적으로 관리하는 기술 개발이 홉 대량증식에서 중요할 것으로 판단된다. 조직배양 기술을 통해 홉을 증식할 땐 IAA 0.1 ㎎/L에 BAP 1 ㎎/L 보다 TDZ 1 ㎎/L를 첨가하여 배양하는 것이 다경유도에 효과적이다. 이러한 삽목 및 조직배양 증식과 순화 기술을 정립하면, 국내 홉 건전묘 생산에 활용이 가능할 것으로 생각된다.

Keywords

Acknowledgement

본 연구는 한국연구재단(NSF) 지원의 지역대학우수과학자지원사업 연구과제(No. RS-2022-00156231) 의 일부로 수행되었으며, 지원에 감사를 표한다.

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