Molecules and Cells

Korean Society for Molecular and Cellular Biology (한국분자세포생물학회)
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Determinants of Functional MicroRNA Targeting

  • Hyeonseo Hwang (School of Biological Sciences, Seoul National University) ;
  • Hee Ryung Chang (School of Biological Sciences, Seoul National University) ;
  • Daehyun Baek (School of Biological Sciences, Seoul National University)
  • Received : 2022.10.14
  • Accepted : 2022.11.15
  • Published : 2023.01.31
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Abstract

MicroRNAs (miRNAs) play cardinal roles in regulating biological pathways and processes, resulting in significant physiological effects. To understand the complex regulatory network of miRNAs, previous studies have utilized massivescale datasets of miRNA targeting and attempted to computationally predict the functional targets of miRNAs. Many miRNA target prediction tools have been developed and are widely used by scientists from various fields of biology and medicine. Most of these tools consider seed pairing between miRNAs and their mRNA targets and additionally consider other determinants to improve prediction accuracy. However, these tools exhibit limited prediction accuracy and high false positive rates. The utilization of additional determinants, such as RNA modifications and RNA-binding protein binding sites, may further improve miRNA target prediction. In this review, we discuss the determinants of functional miRNA targeting that are currently used in miRNA target prediction and the potentially predictive but unappreciated determinants that may improve prediction accuracy.

Keywords

Acknowledgement

This study was supported by the National Research Foundation of Korea (NRF), which is funded by the Ministry of Science and ICT, Republic of Korea (NRF-2014M3C9A3063541, NRF-2019M3E5D3073104, NRF-2020R1A2C3007032, NRF2020R1A5A1018081, and NRF-2022M3A9I2082294), the Korea Health Industry Development Institute (KHIDI), which is funded by the Ministry of Health and Welfare, Republic of Korea (HI15C3224), and the Korea National Institute of Health (KNIH) which is funded by the Korea Disease Control and Prevention Agency (KDCA) (2022-ER1605-00).

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