Journal of Plant Biotechnology

  • Volume 50
  • /
  • Pages.108-114
  • /
  • 2023
  • /
  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effects of plant preservative mixtureTM on in vitro germination of Dendrobium thyrsiflorum Rchb.f. and its application in orchid conservation

  • Tran Trung Chanh (Research Center for High-Tech Application in Agriculture (RCHAA), University of Science, Vietnam National University) ;
  • Nguyen Tan Huy (Research Center for High-Tech Application in Agriculture (RCHAA), University of Science, Vietnam National University) ;
  • Nguyen Thu Ha (Research Center for High-Tech Application in Agriculture (RCHAA), University of Science, Vietnam National University) ;
  • Khanh Le (Cisbay Global Inc.) ;
  • Nguyen Huu Hoang (Research Center for High-Tech Application in Agriculture (RCHAA), University of Science, Vietnam National University)
  • Received : 2023.04.15
  • Accepted : 2023.05.11
  • Published : 2023.06.05
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Abstract

In vitro conservation is one of the most effective strategies for rare plant protection, especially for orchid species. To maximize the success rates of in vitro explant establishment (stage I) in conservation programs, the application of tissue culture additives such as Plant Preservative MixtureTM (PPMTM) should be emphasized. In this study, we used Dendrobium thyrsiflorum Rchb.f. (1875) seeds and seedlings as a model for the evaluation of PPMTM's phytotoxicity in the meristematic tissues of epiphytic orchids. PPMTM had no observable inhibitory effect on protocorm, shoot, or root development when it was supplemented at 0.1%. PPMTM supplementation caused adverse effects on D. thyrsiflorum explants at concentrations > 0.2%. At high concentrations, young in vitro seedlings showed damage, especially at the root tissue level. Based on this model, supplementation of 0.1-0.2% PPMTM to culture media was successfully implemented to establish in vitro cultures of other rare orchid species in our conservation program.

Keywords

Acknowledgement

This research is funded by Vietnam National University, Ho Chi Minh City (VNU-HCM) under grant number C2020-18-02.

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