Journal of Plant Biotechnology

  • 제50권
  • /
  • Pages.82-88
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  • 2023
  • /
  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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무 원종 대량생산을 위한 경정배양

Shoot-tip culture for massive production of radish foundation seeds

  • 박한용 (세종대학교 바이오산업자원공학과) ;
  • 김유경 (세종대학교 바이오산업자원공학과) ;
  • 최수빈 (세종대학교 바이오산업자원공학과) ;
  • 모숙연 (세종대학교 바이오산업자원공학과)
  • Han Yong Park (Department of Bioengineering, Sejong University) ;
  • You Kyoung Kim (Department of Bioengineering, Sejong University) ;
  • Soo Bin Choi (Department of Bioengineering, Sejong University) ;
  • Sug Youn Mo (Department of Bioengineering, Sejong University)
  • 투고 : 2023.02.08
  • 심사 : 2023.04.17
  • 발행 : 2023.05.15
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초록

순도가 균일한 원종을 대량생산하기 위해 원원종 종자를 이용하여 조직배양 기술 중 경정배양을 이용하였다. 실험 재료로는 원원종 계통 RA2와 RA4 계통을 사용하였다. BA 호르몬 사용의 경우 RA2 계통의 유묘에서는 BA 1.33 uM 첨가 배지에서 평균 14.67개로, 성묘의 경우 BA 1.78 uM 첨가 배지에서 평균 11.33개로 가장 많은 개수의 multi-shoots이 형성되었으며, RA4 계통 경우 유묘에서는 BA 2.22 uM 첨가 배지에서 평균 11.67개로, 성묘에서는 BA 1.33 uM 첨가 배지에서 평균 13.67개로 가장 많은 multi-shoots이 형성되었지만 BA 농도별로 유의수준에서 거의 차이가 없는 것으로 나타났다. TDZ 호르몬 사용의 경우 RA2 계통은 TDZ 농도가 증가할수록 multi-shoots의 수가 증가하여 TDZ 0.45 uM 첨가 배지에서 가장 많은 multi-shoots가 형성되었으나(유묘 7.0개, 성묘 3.0개), TDZ 2.25 uM 부터는 거의 multi-shoots가 형성되지 않았다. RA4 계통은 유묘의 경우 거의 multi-shoots이 생성되지 않았고 성묘의 경우 TDZ 0.23, 0.45 uM 첨가 배지에서 3.7개의 multi-shoots이 생성되었으나 그 이상의 농도에서는 유묘에서와 같이 생성되지 않았다. 생성된 multi-shoots를 계대배양하여 생장한 조직배양묘를 SSR marker를 사용하여 원종계통과 비교분석한 결과 생성된 조직배양묘에서 변이의 양상이 약하게 보인 것이 있었으나 돌연변이체로 나타난 것은 없었다. 또한 뿌리 발근에 있어서는 RA2, RA4 계통 모두 대체적으로 IBA 4.9 uM 첨가된 배지에서 발근율, 뿌리 수, 뿌리발달 정도 등이 가장 좋았다.

Shoot-tip culture was used to produce clonal plants of radish stock seeds. Using 6-benzyladenine (BA), the largest number of RA2 line multi-shoots were formed with an average of 14.67 shoots on 1.33 µM BA in early seedlings and 11.33 shoots on 1.78 µM BA in juvenile seedlings. The largest number of RA4 line multi-shoots were formed with an average of 11.67 shoots on 2.22 µM BA in early seedlings and 13.67 shoots on 1.33 µM BA in juvenile seedlings. There was little difference in the significance level by BA concentration in both lines. Using Thidiazuron (TDZ), the number of RA2 line multi-shoots increased with increasing TDZ concentration, forming the largest number of multi-shoots in 0.45 µM TDZ (7.0 and 3.0 multi-shoots for early and juvenile seedlings, respectively), but few multi-shoots were formed from TDZ 2.25 and 4.5 µM. RA4 line produced almost no multi-shoots in early seedlings, and 3.7 multi-shoots were produced in 0.23 and 0.45 µM TDZ in juvenile seedlings, but not at higher concentrations. Analysis of the tissue culture seedlings grown by cultivating the generated multi-shoots with Radish Foundation seeds using SSR marker revealed a weak pattern of mutation in the generated tissue culture seedlings, but there was no mutant. In addition, in terms of root roots, both RA2 and RA4 lines generally had the best rooting, number of roots, and degree of root development in 4.9 µM indol-3- butyric acid (IBA).

키워드

과제정보

본 결과물은 농촌진흥청의 재원으로 국가생명연구자원선진화사업의 지원을 받아 연구되었음(PJ016045032023).

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