Journal of Applied Biological Chemistry

  • Volume 66
  • /
  • Pages.171-178
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  • 2023
  • /
  • 1976-0442(pISSN)
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  • 2234-7941(eISSN)
The Korean Society for Applied Biological Chemistry (한국응용생명화학회)
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Pharmacological actions of morusinol on modulation of platelet functions via integrin αIIb/β3 signaling

  • Hyuk-Woo Kwon (Department of Biomedical Laboratory Science and Microbiological Resource Research Institute, Far East University)
  • Received : 2023.02.24
  • Accepted : 2023.04.17
  • Published : 2023.12.31
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Abstract

Morus alba, a popular medicinal plant belonging to the family Moraceae, has long been used commonly in traditional medicine and has various physiological activities, including antidiabetic, anti-microbial, diuretic, anti-oxidant, and anti-cancer activities. Morusinol was isolated from the root bark of M. alba; however, its biological effects have not yet been reported. Therefore, we examined the inhibitory effects of morusinol on human platelet aggregation, Ca2+ mobilization, and αIIb/β3 activity. Our data showed that collagen-induced human platelet aggregation was inhibited by morusinol without cytotoxicity. In this study, we examined whether morusinol inhibits platelet aggregation through the regulation of integrin αIIb/β3 and its associated signaling molecules. We observed that morusinol inhibited αIIb/β3 activation by regulating vasodilator-stimulated phosphoprotein, phosphatidylinositol-3 kinase, Akt (protein kinase B), and glycogen synthase kinase-3α/β. These results show that morusinol inhibited fibronectin adhesion, fibrinogen binding, and clot retraction. Taken together, morusinol shows strong antiplatelet and anti-clot retraction effects and is a potential therapeutic drug candidate to prevent platelet-related thrombosis and cardiovascular disease.

Keywords

Acknowledgement

This work was supported by a 2022 Far East University Research Grant (FEU2022S04).

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