Clinical and Experimental Reproductive Medicine

The Korean Society for Reproductive Medicine (대한생식의학회)
권호 표지
DOI QR코드

DOI QR Code

Clinical and laboratory factors associated with the presence of dysmorphic oocytes in intracytoplasmic sperm injection cycles

  • Tae Eun Kim (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) ;
  • Hyun Kyung Lee (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital) ;
  • Byung Chul Jee (Department of Obstetrics and Gynecology, Seoul National University Bundang Hospital)
  • Received : 2023.03.20
  • Accepted : 2023.07.10
  • Published : 2023.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Objective: This study investigated the clinical and laboratory factors associated with the presence of dysmorphic oocytes in intracytoplasmic sperm injection (ICSI) cycles. Methods: The study involved 200 ICSI cycles, performed from 2020 to 2021, that yielded at least one mature oocyte. Clinical characteristics and ovarian stimulation methods were compared between 68 cycles with at least one dysmorphic oocyte (the dysmorphic group) and 132 cycles with normal-form oocytes only (the non-dysmorphic group). Dysmorphic oocytes were characterized by dark cytoplasm, cytoplasmic granularity, cytoplasmic vacuoles, refractile bodies in the cytoplasm, smooth endoplasmic reticulum in the cytoplasm, an oval shape, an abnormal zona pellucida, a large perivitelline space, debris in the perivitelline space, or an abnormal polar body. Results: The ages of the women, indications for in vitro fertilization, serum anti-Müllerian hormone levels, and rates of current ovarian endometrioma were similar between the dysmorphic and non-dysmorphic groups. In both groups, the three ovarian stimulation regimens, two types of pituitary suppression, and total gonadotropin dose were employed similarly. However, the dual-trigger method was used more frequently in the dysmorphic group (67.6% vs. 50%, p=0.024). The dysmorphic group contained significantly more immature oocytes and exhibited significantly lower oocyte maturity (50% vs. 66.7%, p=0.001) than the non-dysmorphic cycles. Within the dysmorphic group, significantly lower oocyte maturity was found in the cycles using a dual-trigger, but not in those with a human chorionic gonadotropin trigger. Conclusion: ICSI cycles with dysmorphic oocytes are closely associated with reduced oocyte maturity. This association was observed exclusively in dual-trigger cycles.

Keywords

References

  1. Lasiene K, Vitkus A, Valanciute A, Lasys V. Morphological criteria of oocyte quality. Medicina (Kaunas) 2009;45:509-15.  https://doi.org/10.3390/medicina45070067
  2. Rienzi L, Vajta G, Ubaldi F. Predictive value of oocyte morphology in human IVF: a systematic review of the literature. Hum Reprod Update 2011;17:34-45.  https://doi.org/10.1093/humupd/dmq029
  3. Ozturk S. Selection of competent oocytes by morphological criteria for assisted reproductive technologies. Mol Reprod Dev 2020;87:1021-36.  https://doi.org/10.1002/mrd.23420
  4. Yu EJ, Ahn H, Lee JM, Jee BC, Kim SH. Fertilization and embryo quality of mature oocytes with specific morphological abnormalities. Clin Exp Reprod Med 2015;42:156-62.  https://doi.org/10.5653/cerm.2015.42.4.156
  5. De Sutter P, Dozortsev D, Qian C, Dhont M. Oocyte morphology does not correlate with fertilization rate and embryo quality after intracytoplasmic sperm injection. Hum Reprod 1996;11:595-7.  https://doi.org/10.1093/HUMREP/11.3.595
  6. Coello A, Sanchez E, Vallejo B, Meseguer M, Remohi J, Cobo A. Effect of oocyte morphology on post-warming survival and embryo development in vitrified autologous oocytes. Reprod Biomed Online 2019;38:313-20.  https://doi.org/10.1016/j.rbmo.2018.11.028
  7. Verlinsky Y, Lerner S, Illkevitch N, Kuznetsov V, Kuznetsov I, Cieslak J, et al. Is there any predictive value of first polar body morphology for embryo genotype or developmental potential? Reprod Biomed Online 2003;7:336-41.  https://doi.org/10.1016/S1472-6483(10)61874-3
  8. De Santis L, Cino I, Rabellotti E, Calzi F, Persico P, Borini A, et al. Polar body morphology and spindle imaging as predictors of oocyte quality. Reprod Biomed Online 2005;11:36-42.  https://doi.org/10.1016/S1472-6483(10)61296-5
  9. Kahraman S, Yakin K, Donmez E, Samli H, Bahce M, Cengiz G, et al. Relationship between granular cytoplasm of oocytes and pregnancy outcome following intracytoplasmic sperm injection. Hum Reprod 2000;15:2390-3. https://doi.org/10.1093/humrep/15.11.2390
  10. Ebner T, Moser M, Tews G. Is oocyte morphology prognostic of embryo developmental potential after ICSI? Reprod Biomed Online 2006;12:507-12.  https://doi.org/10.1016/S1472-6483(10)62006-8
  11. Ten J, Mendiola J, Vioque J, de Juan J, Bernabeu R. Donor oocyte dysmorphisms and their influence on fertilization and embryo quality. Reprod Biomed Online 2007;14:40-8. 
  12. Rienzi L, Ubaldi FM, Iacobelli M, Minasi MG, Romano S, Ferrero S, et al. Significance of metaphase II human oocyte morphology on ICSI outcome. Fertil Steril 2008;90:1692-700.  https://doi.org/10.1016/j.fertnstert.2007.09.024
  13. Braga DP, Setti AS, Figueira Rde C, Machado RB, Iaconelli A Jr, Borges E Jr. Influence of oocyte dysmorphisms on blastocyst formation and quality. Fertil Steril 2013;100:748-54.  https://doi.org/10.1016/j.fertnstert.2013.05.021
  14. de Cassia S Figueira R, de Almeida Ferreira Braga DP, Semiao-Francisco L, Madaschi C, Iaconelli A Jr, Borges E Jr. Metaphase II human oocyte morphology: contributing factors and effects on fertilization potential and embryo developmental ability in ICSI cycles. Fertil Steril 2010;94:1115-7.  https://doi.org/10.1016/j.fertnstert.2009.11.039
  15. Halvaei I, Ali Khalili M, Razi MH, Nottola SA. The effect of immature oocytes quantity on the rates of oocytes maturity and morphology, fertilization, and embryo development in ICSI cycles. J Assist Reprod Genet 2012;29:803-10.  https://doi.org/10.1007/s10815-012-9799-6
  16. Azizi E, Naji M, Nazari L, Salehpour S, Karimi M, Borumandnia N, et al. Serum anti-Mullerian hormone is associated with oocyte dysmorphisms and ICSI outcomes. Int J Gynaecol Obstet 2019;147:179-86.  https://doi.org/10.1002/ijgo.12941
  17. Sigala J, Sifer C, Dewailly D, Robin G, Bruyneel A, Ramdane N, et al. Is polycystic ovarian morphology related to a poor oocyte quality after controlled ovarian hyperstimulation for intracytoplasmic sperm injection?: results from a prospective, comparative study. Fertil Steril 2015;103:112-8.  https://doi.org/10.1016/j.fertnstert.2014.09.040
  18. Robin C, Uk A, Decanter C, Behal H, Collinet P, Rubod C, et al. Impact of endometriosis on oocyte morphology in IVF-ICSI: retrospective study of a cohort of more than 6000 mature oocytes. Reprod Biol Endocrinol 2021;19:160. 
  19. Kim SJ, Kim TH, Park JK, Eum JH, Lee WS, Lyu SW. Effect of a dual trigger on oocyte maturation in young women with decreased ovarian reserve for the purpose of elective oocyte cryopreservation. Clin Exp Reprod Med 2020;47:306-11.  https://doi.org/10.5653/cerm.2020.03657
  20. Hong YH, Kim SK, Lee JR, Jee BC, Suh CS. Clinical efficacy of dual trigger with human chorionic gonadotropin and a gonadotropin-releasing hormone agonist for women undergoing fertility preservation. Reprod Med Biol 2022;21:e12440. 
  21. Ding N, Liu X, Jian Q, Liang Z, Wang F. Dual trigger of final oocyte maturation with a combination of GnRH agonist and hCG versus a hCG alone trigger in GnRH antagonist cycle for in vitro fertilization: a systematic review and meta-analysis. Eur J Obstet Gynecol Reprod Biol 2017;218:92-8. https://doi.org/10.1016/j.ejogrb.2017.09.004