DOI QR코드

DOI QR Code

Phase Differences Averaging (PDA) Method for Reducing the Phase Error in Digital Holographic Microscopy (DHM)

  • Hyun-Woo, Kim (Department of Computer Science and Networks, Kyushu Institute of Technology) ;
  • Jaehoon, Lee (Department of Computer Science and Networks, Kyushu Institute of Technology) ;
  • Arun, Anand (Department of Physics, Sardar Patel University) ;
  • Myungjin, Cho (School of ICT, Robotics, and Mechanical Engineering, IITC, Hankyong National University) ;
  • Min-Chul, Lee (Department of Computer Science and Networks, Kyushu Institute of Technology)
  • Received : 2022.08.08
  • Accepted : 2022.10.14
  • Published : 2023.03.31

Abstract

Digital holographic microscopy (DHM) is a three-dimensional (3D) imaging technique that uses the phase information of coherent light. In the reconstruction process of DHM, a narrow region around the positive or negative sideband from the Fourier domain is windowed to avoid noise due to the DC spectrum of the hologram spectrum. However, the limited size of the window also degrades the high-frequency information of the 3D object profile. Although a large window can have more detailed information of the 3D object shape, the noise is increased. To solve this trade-off, we propose phase difference averaging (PDA). The proposed method yields high-frequency information of the specimen while reducing the DC noise. In this paper, we explain the reconstruction algorithm for this method and compare it to various conventional filtering methods including Gaussian, Wiener, average, median, and bilateral filtering methods.

Keywords

Acknowledgement

This work was supported by the Japan-Korea Basic Scientific Cooperation Program between JSPS and NRF, Grant number (JPJSBP 120228811), and this work was supported under the framework of the international cooperation program managed by the National Research Foundation of Korea(NRF-2022K2A9A2A08000152, FY2022).

References

  1. D. Gabor, "A new microscopic principle," Nature, vol. 161, pp. 777-778, May. 1948. DOI: 10.1038/161777a0.
  2. T. Pitkaaho, A. Manninen, and T. J. Naughton, "Focus prediction in digital holographic microscopy using deep convolutional neural networks," Applied Optics, vol. 58, no. 5, pp. A202-A208, Jan. 2019. DOI: 10.1364/AO.58.00A202.
  3. J. A. Picazo-Bueno, M. Trusiak, and V. Mico, "Single-shot slightly off-axis digital holographic microscopy with add-on module based on beamsplitter cube," Optics Express, vol. 27, no. 4, pp. 5655-5669, Feb. 2019. DOI: 10.1364/OE.27.005655.
  4. S. Shin and Y. Yu, "Lensless reflection digital holographic microscope with a fresnel-bluestein transform," Journal of the Korean Physical Society, vol. 74, no. 2, pp. 98-101, Jan. 2019. DOI: 10.3938/jkps.74.98.
  5. T. O'Connor, A. Doblas, and B. Javidi, "Structured illumination in compact and field-portable 3D-printed shearing digital holographic microscopy for resolution enhancement," Optics Letters, vol. 44, no. 9, pp. 2326-2329, May. 2019. DOI: 10.1364/OL.44.002326.
  6. Z. Zhong, H. Zhao, L. Cao, M. Shan, B. Liu, W. Lu, and H. Xie, "Automatic cross filtering for off-axis digital holographic microscopy," Results in Physics, vol. 16, p. 102910, Mar. 2020. DOI: 10.1016/j.rinp.2019.102910.
  7. T. O'Connor, A. Anand, and B. Javidi, "Field-portable microsphereassisted high resolution digital holographic microscopy in compact and 3D-printed Mach-Zehnder Interferometer," OSA Continuum, vol. 3, no. 4, pp. 1013-1020, Apr. 2020. DOI: 10.1364/OSAC.389832.
  8. J. Dong, A. K. Yetisen, X. Dong, F. Poller, M. Jakobi, Z. Liu, F. S. Bloise, and A. W. Koch, "Low-pass filtering compensation in common-path digital holographic microscopy," Applied Physics Letters, vol. 117, no. 12, p. 121105, Sep. 2020. DOI: 10.1063/5.0019209.
  9. L. Huang, L. Yan, B. Chen, Y. Zhou, and T. Yang, "Phase aberration compensation of digital holographic microscopy with curve fitting preprocessing and automatic background segmentation for microstructure testing," Optics Communications, vol. 462, p. 125311, May. 2020. DOI: 10.1016/j.optcom.2020.125311.
  10. H. W. Kim, K. Inoue, M. Cho, and M. C. Lee, "A study on real-time modification of the refractive index of a surrounding medium using a uniform microsphere in digital holographic microscopy," in Proceedings of the 2020 3rd International Conference on Electronics and Electrical Engineering Technology, Kitakyushu, Japan, pp. 44-48, 2020. DOI: 10.1145/3429536.3429539.
  11. M. L. Piao, H. Y. Wu, and N. Kim, "3D image encryption based on computer-generated hologram," in Digital Holography and Three-Dimensional Imaging. Optical Society of America, Bordeaux, France, pp. W3A-21, 2019. DOI: 10.1364/DH.2019.W3A.21.
  12. I. Shevkunov, V. Katkovnik, D. Claus, G. Pedrini, N. V. Petrov, and K. Egiazarian, "Spectral object recognition in hyperspectral holography with complex-domain denoising," Sensors, vol. 19, no. 23, p. 5188, Nov. 2019. DOI: 10.3390/s19235188.
  13. A. Anand, V. K. Chhaniwal, N. R. Patel, and B. Javidi, "Automatic identification of malaria-infected RBC with digital holographic microscopy using correlation algorithms," IEEE Photonics Journal, vol. 4, no. 5, pp. 1456-1464, Oct. 2012. DOI: 10.1109/JPHOT.2012.2210199.
  14. F. Yi, I. Moon, and Y. H. Lee, "Three-dimensional counting of morphologically normal human red blood cells via digital holographic microscopy," Journal of Biomedical Optics, vol. 20, no. 1, p. 016005, Jan. 2015. DOI: 10.1117/1.JBO.20.1.016005.
  15. B. Kemper and G. Von Bally, "Digital holographic microscopy for live cell applications and technical inspection," Applied Optics, vol. 47, no. 4, pp. A52-A61, Feb. 2008. DOI: 10.1364/AO.47.000A52.
  16. T. Colomb, N. Pavillon, J. Kuhn, E. Cuche, C. Depeursinge, and Y. Emery, "Extended depth-of-focus by digital holographic microscopy," Optics Letters, vol. 35, no. 11, pp. 1840-1842, Jun. 2010. DOI: 10.1364/OL.35.001840.
  17. S. Shin and Y. Yu, "Fine metal mask 3-dimensional measurement by using scanning digital holographic microscope," Journal of the Korean Physical Society, vol. 72, no. 8, pp. 863-867, Apr. 2018. DOI: 10.3938/jkps.72.863.
  18. J. Zakrisson, S. Schedin, and M. Andersson, "Cell shape identification using digital holographic microscopy," Applied Optics, vol. 54, no. 24, pp. 7442-7448, Aug. 2015. DOI: 10.1364/AO.54.007442.
  19. D. Roitshtain, N. A. Turko, B. Javidi, and N. T. Shaked, "Flipping interferometry and its application for quantitative phase microscopy in a micro-channel," Optics Letters, vol. 41, no. 10, pp. 2354-2357, May. 2016. DOI: 10.1364/OL.41.002354.
  20. A. Anand, V. Chhaniwal, and B. Javidi. "Tutorial: common path selfreferencing digital holographic microscopy," APL Photonics, vol. 3, no. 7, p. 071101, Jul. 2018. DOI: 10.1063/1.5027081.
  21. R. M. Goldstein, H. A. Zebker, and C. L. Werner, "Satellite radar interferometry: Two-dimensional phase unwrapping," Radio Science, vol. 23, no. 4, pp. 713-720, Jul. 1988. DOI: 10.1029/RS023i004p00713.
  22. R. C. Gonzalez, and R. E. Woods, Digital image processing, 4th ed., New York, NY: Pearson, 2002.