Molecules and Cells

  • 제46권11호
  • /
  • Pages.710-724
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  • 2023
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  • 1016-8478(pISSN)
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  • 0219-1032(eISSN)
한국분자세포생물학회 (Korean Society for Molecular and Cellular Biology)
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The Ralstonia pseudosolanacearum Type III Effector RipL Delays Flowering and Promotes Susceptibility to Pseudomonas syringae in Arabidopsis thaliana

  • Wanhui Kim (Plant Immunity Research Center, Seoul National University) ;
  • Hyelim Jeon (Plant Immunity Research Center, Seoul National University) ;
  • Hyeonjung Lee (School of Interdisciplinary Bioscience and Bioengineering, Pohang University of Science and Technology) ;
  • Kee Hoon Sohn (Plant Immunity Research Center, Seoul National University) ;
  • Cecile Segonzac (Plant Immunity Research Center, Seoul National University)
  • 투고 : 2023.08.02
  • 심사 : 2023.09.24
  • 발행 : 2023.11.30
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초록

The plant defense responses to microbial infection are tightly regulated and integrated with the developmental program for optimal resources allocation. Notably, the defense-associated hormone salicylic acid (SA) acts as a promoter of flowering while several plant pathogens actively target the flowering signaling pathway to promote their virulence or dissemination. Ralstonia pseudosolanacearum inject tens of effectors in the host cells that collectively promote bacterial proliferation in plant tissues. Here, we characterized the function of the broadly conserved R. pseudosolanacearum effector RipL, through heterologous expression in Arabidopsis thaliana. RipL-expressing transgenic lines presented a delayed flowering, which correlated with a low expression of flowering regulator genes. Delayed flowering was also observed in Nicotiana benthamiana plants transiently expressing RipL. In parallel, RipL promoted plant susceptibility to virulent strains of Pseudomonas syringae in the effector-expressing lines or when delivered by the type III secretion system. Unexpectedly, SA accumulation and SA-dependent immune signaling were not significantly affected by RipL expression. Rather, the RNA-seq analysis of infected RipL-expressing lines revealed that the overall amplitude of the transcriptional response was dampened, suggesting that RipL could promote plant susceptibility in an SA-independent manner. Further elucidation of the molecular mechanisms underpinning RipL effect on flowering and immunity may reveal novel effector functions in host cells.

키워드

과제정보

This work was carried out with the support of the National Research Foundation of Korea (NRF) projects No. 2022R1I1A1A01066399, No. 2019R1I1A1A01060721, and No. 2018R1A5A1023599.

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