Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
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Use of extraction solvent method to monitor the concentrations of acidic polysaccharides and ginsenosides from red and black ginseng

추출용매에 따른 홍삼 및 흑삼의 산성다당체와 진세노사이드 함량 모니터링

  • Gee Dong Lee (Department of Biofood Science and Technology, Joongbu University)
  • 이기동 (중부대학교 바이오식품학전공)
  • Received : 2023.08.22
  • Accepted : 2023.09.24
  • Published : 2023.10.30
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Abstract

In this study, the extraction yield, acidic polysaccharides and ginsenosides of red and black ginseng were optimized by using the response surface methodology in consideration of the ethanol concentration and temperature of the extraction. The R2 of the model formula for the yield, acidic polysaccharides and ginsenosides was 0.8378-0.9679 (p<0.1). An optimal extraction yield of 5.29% was reached for red ginseng soluble solids when 1.52% ethanol concentration was used at a temperature of 67.27℃. Additionally, the optimal extraction yield for black ginseng soluble solid was 6.11% when 3.12% ethanol concentration was used at a temperature of 66.13℃. Furthermore, the optimal conditions for extracting acidic polysaccharides from red ginseng were using an ethanol concentration of 4.03% at a temperature of 69.61℃; a yield of 1.86 mg/mL was obtained. The optimal extraction yield for acidic polysaccharides from black ginseng was 1.80 mg/mL when extracted using a concentration of 24.67% of ethanol at a temperature of 71.14℃. An optimal extraction yield of 0.22 mg/mL was reached for ginsenoside Rg1 from red ginseng when 79.92% ethanol concentration was used at a temperature of 70.62℃. The optimal extraction yield of ginsenoside Rg3 from black ginseng was 0.31 mg/mL when ethanol was used at a concentration of 75.70% at a temperature of 65.49℃. The ideal extraction conditions for obtaining the maximum yield of both acidic polysaccharide and ginsenoside from red and black ginseng were using ethanol at a concentration between 35 and 50% at an extraction temperature of 70℃.

본 연구에서는 홍삼과 흑삼의 기능성 성분 추출 극대화를 위해 추출 용액의 ethanol 농도와 추출 온도를 고려하여 추출 수율, 산성다당체 및 ginsenosides의 함량 변화를 반응표면분석법을 통해 모니터링해 보고 적정 추출조건을 찾아보았다. 홍삼 및 흑삼의 가용성 고형분 함량에 대한 모델식의 R2는 각각 0.9679(p<0.01), 0.8545(p<0.1)였다. 홍삼가용성 고형분의 최적 추출조건은 ethanol 농도 1.52%에서 67.27℃로 추출 시 그 함량이 5.29%였으며, 흑삼 가용성 고형분의 최적 추출조건은 ethanol 농도 3.12%에서 66.13℃로 추출 시 그 함량이 6.11%였다. 홍삼 및 흑삼의 산성다당체 함량에 대한 모델식의 R2는 각각 0.9251(p<0.05), 0.88379(p<0.1)였다. 홍삼의 산성다당체 최적 추출조건은 ethanol 농도 4.03%에서 69.61℃로 추출 시 그 함량이 1.86 mg/mL였다. 흑삼의 산성다당체 최적 추출조건은 ethanol 용액 농도 24.67%에서 71.14℃로 추출 시 그 함량이 1.80 mg/mL였다. 홍삼의 ginsenoside Rg1 및 Rb1 함량에 대한 모델식의 R2는 각각 0.8941(p<0.05), 0.8718(p<0.1)이었다. 홍삼의 ginsenosides 최적 추출조건은 ethanol 농도 79.92%에서 70.62℃로 추출 시 ginsenoside Rg1 함량이 0.22 mg/mL였으며, ethanol 농도 79.94%에서 69.46℃에서 ginsenoside Rb1 함량이 0.36 mg/mL였다. 흑삼의 ginsenosides 최적 추출조건은 ethanol 농도 75.11%에서 65.21℃로 추출할 경우 ginsenoside Rb1 함량이 0.28 mg/mL였으며, ethanol 농도 75.70%에서 65.49℃에서 ginsenoside Rg3 함량이 0.31 mg/mL였다. 홍삼 및 흑삼의 산성다당체 수율과 ginsenoside 수율을 모두 만족하는 최적추출조건은 ethanol 농도 35-50%의 범위 내에서 70℃였다.

Keywords

Acknowledgement

이 논문은 2023년 중부대학교 학술연구비 지원으로 이루어진 것입니다. 이에 감사드립니다.

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