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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Nonsense-mediated mRNA decay, a simplified view of a complex mechanism

  • Julie Carrard (Univ. Lille, CNRS, Inserm, UMR9020-U1277 - CANTHER - Cancer Heterogeneity Plasticity and Resistance to Therapies) ;
  • Fabrice Lejeune (Univ. Lille, CNRS, Inserm, UMR9020-U1277 - CANTHER - Cancer Heterogeneity Plasticity and Resistance to Therapies)
  • Received : 2023.09.15
  • Accepted : 2023.11.17
  • Published : 2023.12.31
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Abstract

Nonsense-mediated mRNA decay (NMD) is both a quality control mechanism and a gene regulation pathway. It has been studied for more than 30 years, with an accumulation of many mechanistic details that have often led to debate and hence to different models of NMD activation, particularly in higher eukaryotes. Two models seem to be opposed, since the first requires intervention of the exon junction complex (EJC) to recruit NMD factors downstream of the premature termination codon (PTC), whereas the second involves an EJC-independent mechanism in which NMD factors concentrate in the 3'UTR to initiate NMD in the presence of a PTC. In this review we describe both models, giving recent molecular details and providing experimental arguments supporting one or the other model. In the end it is certainly possible to imagine that these two mechanisms co-exist, rather than viewing them as mutually exclusive.

Keywords

Acknowledgement

JC is supported by Inca. FL is supported by funding from ANR, Inca, La Ligue contre le cancer, Vaincre la mucoviscidose and the Association Francaise contre les Myopathies. The Canther Laboratory is part of the ONCOLille institute. This work is supported by a grant from Contrat de Plan Etat-Region CPER Cancer 2015-2020.

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