대한한의학방제학회지 (Herbal Formula Science)

  • 제31권4호
  • /
  • Pages.295-313
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  • 2023
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  • 1229-1218(pISSN)
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  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
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시스템 약리학적 분석에 의한 상산의 암전이 억제 효과

Systems Pharmacological Analysis of Dichroae Radix in Anti-Tumor Metastasis Activity

  • 이지예 (부산대학교 한의학전문대학원) ;
  • 신아연 (부산대학교 한의학전문대학원) ;
  • 김학군 (부산대학교 한의학전문대학원) ;
  • 안원근 (부산대학교 한의학전문대학원)
  • Jee Ye Lee (School of Korean Medicine, Pusan National University) ;
  • Ah Yeon Shin (School of Korean Medicine, Pusan National University) ;
  • Hak Koon Kim (School of Korean Medicine, Pusan National University) ;
  • Won Gun An (School of Korean Medicine, Pusan National University)
  • 투고 : 2023.11.01
  • 심사 : 2023.11.13
  • 발행 : 2023.11.30
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초록

Objectives : While treatments for cancer are advancing, the development of effective treatments for cancer metastasis, the main cause of cancer patient death, remains insufficient. Recent studies on Dichroae Radix have revealed that its active ingredients have the potential to inhibit cancer metastasis. This study aimed to investigate the cancer metastasis inhibitory effect of Dichroae Radix using network pharmacological analysis. Methods : The active compounds of Dichroae Radix have been identified using Traditional Chinese Medicine System Pharmacology Database and Analysis Platform. The UniProt database was used to collect each of information of all target proteins associated with the active compounds. To find the bio-metabolic processes associated with each target, the DAVID6.8 Gene Functional classifier tool was used. Compound-Target and Target-Pathway networks were analyzed via Cytoscape 3.40. Results : In total, 25 active compounds and their 62 non-redundant targets were selected through the TCMSP database and analysis platform. The target genes underwent gene ontology and pathway enrichment analysis. The gene list applied to the gene ontology analysis revealed associations with various biological processes, including signal transduction, chemical synaptic transmission, G-protein-coupled receptor signaling pathways, response to xenobiotic stimulus, and response to drugs, among others. A total of eleven genes, including HSP90AB1, CALM1, F2, AR, PAKACA, PTGS2, NOS2, RXRA, ESR1, ESR2, and NCOA1, were found to be associated with biological pathways related to cancer metastasis. Furthermore, nineteen of the active compounds from Dichroae Radix were confirmed to interact with these genes. Conclusions : The results provide valuable insights into the mechanism of action and molecular targets of Dichroae Radix. Notably, Berberine, the main active ingredient of Dichroae Radix, plays a significant role in degrading AR proteins in advanced prostate cancer. Further studies and validations can provide crucial data to advance cancer metastasis prevention and treatment strategies.

키워드

과제정보

This work was supported by a 2-Years Research Grant of Pusan National University.

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