Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Ukgan-san plus Citri Pericarpium and Pinelliae Rhizoma Protects Hepatocytes from Arachidonic Acid and Iron-mediated Oxidative Stress

아라키돈산과 철 유도성 산화적 스트레스에 대한 억간산가진피반하(抑肝散加陳皮半夏)의 간세포 보호 효능

  • Ye Lim Kim (College of Korean Medicine, Daegu Haany University) ;
  • Hyo Jeong Jin (College of Korean Medicine, Daegu Haany University) ;
  • Sang Mi Park (College of Korean Medicine, Daegu Haany University) ;
  • Kyung Hwan Jegal (College of Korean Medicine, Daegu Haany University) ;
  • Chang Hyun Song (College of Korean Medicine, Daegu Haany University) ;
  • Kyung Soon Kim (College of Korean Medicine, Daegu Haany University) ;
  • Sung Hui Byun (College of Korean Medicine, Daegu Haany University) ;
  • Sang Chan Kim (College of Korean Medicine, Daegu Haany University)
  • 김예림 (대구한의대학교 한의과대학) ;
  • 진효정 (대구한의대학교 한의과대학) ;
  • 박상미 (대구한의대학교 한의과대학) ;
  • 제갈경환 (대구한의대학교 한의과대학) ;
  • 송창현 (대구한의대학교 한의과대학) ;
  • 김경순 (대구한의대학교 한의과대학) ;
  • 변성희 (대구한의대학교 한의과대학) ;
  • 김상찬 (대구한의대학교 한의과대학)
  • Received : 2023.09.25
  • Accepted : 2023.11.12
  • Published : 2023.11.30
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Abstract

Objectives : Ukgan-san plus Citri Pericarpium and Pinelliae Rhizoma (UCP) is used as a traditional herbal formula in Korea and Japan for treatment of fever, fever-induced convulsions, and liver dysfunction and so on. In this study, we investigated the cytoprotective effect and underlying mechanism of UCP against oxidative stress induced by cotreatment of arachidonic acid (AA) and iron. Methods : To evaluate the hepatoprotective effects of UCP against AA + iron-induced oxidative stress in HepG2 cell, cell viability and changes on apoptosis-related proteins were assessed by MTT and immunoblot analyses. The changes in intracellular reactive oxygen species (ROS), glutathione (GSH), and mitochondrial membrane permeability (MMP) were investigated against to the oxidative stress. Furthermore, to verify underlying molecular mechanism, NF-E2-related factor 2 (Nrf2) and its downstream target genes were examined by immunoblot analysis. Results : Treatment of UCP increased the cell viability and altered the expression levels of apoptosis-related proteins such as PARP, caspase-9, caspase-3, Bcl-2. UCP also inhibited the GSH depletion, excessive ROS production and mitochondrial dysfunction induced by AA + iron. In addition, the Nrf2 and the Nrf2 target genes activation were increased by UCP. Conclusions : These results indicated that UCP has the ability to protect against oxidative stress-induced hepatocyte damage, which may be mediated with Nrf2 pathway.

Keywords

Acknowledgement

This study was supported by the National Research Foundation of Korea funded by Korea government (MSIP) (Grant No.2018R1A5A2025272)

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