Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
권호 표지
DOI QR코드

DOI QR Code

Effect of suitable dietary glycine supplementation on growth production, meat quality, serum parameters, and stress alleviation of broiler under heat stress condition

  • Jiseon Son (Poultry Research Institute, National Institute of Animal Science, Rural Development Administration) ;
  • Woo-Do Lee (Poultry Research Institute, National Institute of Animal Science, Rural Development Administration) ;
  • Hyunsoo Kim (Poultry Research Institute, National Institute of Animal Science, Rural Development Administration) ;
  • Eui-Chul Hong (Poultry Research Institute, National Institute of Animal Science, Rural Development Administration) ;
  • Hee-Jin Kim (Poultry Research Institute, National Institute of Animal Science, Rural Development Administration) ;
  • Yeon-Seo Yun (Poultry Research Institute, National Institute of Animal Science, Rural Development Administration) ;
  • Hwan Ku Kang (Poultry Research Institute, National Institute of Animal Science, Rural Development Administration)
  • Received : 2023.06.07
  • Accepted : 2023.09.13
  • Published : 2023.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to investigate the productivity, meat quality, blood variables, stress responses, and litter quality of broilers offered feed with different levels of Glycine (Gly) supplementation under heat stress condition. A total of 760 one-day-old Ross 308 broiler males were randomly assigned to one of the four dietary treatment groups: (1) basal diet (control; CON); (2) basal diet + Gly 0.1% (Gly 0.1%); (3) basal diet + Gly 0.2% (Gly 0.2%); and (4) basal diet + Gly 0.3% (Gly 0.3%). The environments for all the treatments groups were maintained according to broiler rearing guidelines from day 1 to day 21, and heat stress condition (32 ± 1℃, 60 ±5%) was created from day 22 to the end. The addition of Gly increased weight gain and affected feed intake (p < 0.05). Gly 0.1% group had higher pH and ferric reducing antioxidant power (FRAP) in the chicken meat and lower heterophil (HE)/lymphocyte (LY) ratio in the blood (p < 0.05). In particular, Gly 0.2% treatment group had lower serum corticosterone level (p < 0.05) than other groups. For jejunum morphology, the addition of Gly 0.2% significantly reduced the depth of the crypts (p < 0.05). However, the addition of Gly did not significantly affect litter quality (p > 0.05). In conclusion, the addition of glycine improved productivity and meat quality, alleviated heat stress, and improved intestinal function. Further studies are needed to determine the optimal level and mechanism of action of the additive when ingested.

Keywords

Acknowledgement

본 연구는 2023년 농촌진흥청 국립축산과학원 전문연구원 과정 지원사업과 농촌진흥청의 공동연구사업(과제번호: PJ01502303)에 의해 이루어진 것으로 이에 감사드립니다

References

  1. Abbasi M, Taheri Mirghaed A, Hoseini SM, Rajabiesterabadi H, Hoseinifar SH, Van Doan H. 2023. Effects of dietary glycine supplementation on growth performance, immunological, and erythrocyte antioxidant parameters in common carp, Cyprinus carpio. Animals 13:412. 
  2. Aslam S, Shukat R, Khan MI, Shahid M. 2020. Effect of dietary supplementation of bioactive peptides on antioxidant potential of broiler breast meat and physicochemical characteristics of nuggets. Food Science of Animal Resources 40:55-73.  https://doi.org/10.5851/kosfa.2019.e82
  3. Aviagen. 2018. Ross 308 Management Handbook. Aviagen, Huntsville, AL, USA. 
  4. Awad EA, Zulkifli I, Soleimani AF, Aljuobori A. 2017. Effects of feeding male and female broiler chickens on low-protein diets fortified with different dietary glycine levels under the hot and humid tropical climate. Italian Journal of Animal Science 16:453-461.  https://doi.org/10.1080/1828051X.2017.1291288
  5. Badakhshan Y, Emadi L, Esmaeili-Mahani S, Nazifi S. 2021. The effect of L-tryptophan on the food intake, rectal temperature, and blood metabolic parameters of 7-day-old chicks during feeding, fasting, and acute heat stress. Iranian Journal of Veterinary Research 22:55-64. 
  6. Bello AU, Idrus Z, Meng GY, Awad EA, Farjam AS. 2017. Gut microbiota and transportation stress response affected by tryptophan supplementation in broiler chickens. Italian Journal of Animal Science 17:107-113.  https://doi.org/10.1080/1828051X.2017.1340814
  7. Benzie IFF, Strain JJ. 1996. The ferric reducing ability of plasma (FRAP) as a measure of "Antioxidant power": The FRAP assay. Analytical Biochemistry 239:70-76.  https://doi.org/10.1006/abio.1996.0292
  8. Bessei W. 2006. Welfare of broilers: A review. World's Poultry Science Journal 62:455-466. https://doi.org/10.1079/WPS2005108
  9. Blois MS. 1958. Antioxidant determinations by the use of a stable free radical. Nature 181:1199-1200.  https://doi.org/10.1038/1811199a0
  10. Buege JA, Aust SD. 1978. Microsomal lipid peroxidation. Methods in Enzymology. 52:302-310.  https://doi.org/10.1016/S0076-6879(78)52032-6
  11. Cardinal KM, de Moraes ML, Andretta I, Schirmann GD, Belote BL, Barrios MA, Santin E, Ribeiro AML. 2019. Growth performance and intestinal health of broilers fed a standard or low-protein diet with the addition of a protease. Revista Brasileira de Zootecnia 48:e20180232. 
  12. Dai SF, Wang LK, Wen AY, Wang LX, Jin GM. 2009. Dietary glutamine supplementation improves growth performance, meat quality and colour stability of broilers under heat stress. British Poultry Science 50:333-340.  https://doi.org/10.1080/00071660902806947
  13. de Freitas Dionizio A, de Souza Khatlab A, Alcalde CR, Gasparino E, Feihrmann AC. 2021. Supplementation with free methionine or methionine dipeptide improves meat quality in broilers exposed to heat stress. Journal of Food Science and Technology 58:205-215.  https://doi.org/10.1007/s13197-020-04530-2
  14. El-Atty A, Hanaa K, Attia KM, Salim IH, Yassein DM, El-Slamony AE. 2020. Effect of glycine supplementation of mandarah laying hens diets on production performance and egg quality. Journal of Animal and Poultry Production 11:583-589.  https://doi.org/10.21608/jappmu.2020.161205
  15. El-Wahab A, Aziza A, El-Adl M. 2015. Impact of dietary excess methionine and lysine with or without addition of L-carnitine on performance, blood lipid profile and litter quality in broilers. Asian Journal of Animal and Veterinary Advances 10:191-202. 
  16. Goel A. 2021. Heat stress management in poultry. Journal of Animal Physiology and Animal Nutrition 105:1136-1145.  https://doi.org/10.1111/jpn.13496
  17. Hu H, Bai X, Wen A, Shah AA, Dai S, Ren Q, Wang S, He S, Wang L. 2016. Assessment of interactions between glutamine and glucose on meat quality, AMPK, and glutamine concentrations in pectoralis major meat of broilers under acute heat stress. Journal of Applied Poultry Research 25:370-378.  https://doi.org/10.3382/japr/pfw021
  18. Hu JY, Mohammed AA, Murugesan GR, Cheng HW. 2022. Effect of a synbiotic supplement as an antibiotic alternative on broiler skeletal, physiological, and oxidative parameters under heat stress. Poultry Science 101:101769. 
  19. Hussein MA, Khattak F, Vervelde L, Athanasiadou S, Houdijk JGM. 2023. Growth performance, caecal microbiome profile, short-chain fatty acids, and litter characteristics in response to placement on reused litter and combined threonine, arginine and glutamine supplementation to juvenile male broiler chickens. Animal Microbiome 5:18. 
  20. Hwangbo J, Choi HC, Park SO, Ryu CM, Park BS, Kang HK, Seo OS, Choi YH. 2013. Diet with soy oil, molasses, methionine, lysine and vitamin C improves the growth performance of broilers exposed to extreme heat stress. Journal of Animal and Veterinary Advances 12:464-471. 
  21. Kim DY, Lim B, Kim JM, Kil DY. 2022. Integrated transcriptome analysis for the hepatic and jejunal mucosa tissues of broiler chickens raised under heat stress conditions. Journal of Animal Science and Biotechnology 13:79. 
  22. Kopec W, Wiliczkiewicz A, Jamroz D, Biazik E, Pudlo A, Hikawczuk T, Skiba T, Korzeniowska M. 2016. Antioxidant status of turkey breast meat and blood after feeding a diet enriched with histidine. Poultry Science 95:53-61.  https://doi.org/10.3382/ps/pev311
  23. Koshchaev I, Mezinova K, Ryadinskaya A, Sorokina N, Chuev S. 2020. Identification of cases of pododermatitis in broiler chickens when feeding a probiotic feed additive. E3S Web of Conferences 210:06023. 
  24. Lee WD, Kothari D, Moon SG, Kim J, Kim KI, Ga GW, Kim YG, Kim SK. 2022. Evaluation of non-fermented and fermented Chinese chive juice as an alternative to antibiotic growth promoters of broilers. Animals 12:2742. 
  25. Magnuson AD, Liu G, Sun T, Tolba SA, Xi L, Whelan R, Lei XG. 2020. Supplemental methionine and stocking density affect antioxidant status, fatty acid profiles, and growth performance of broiler chickens. Journal of Animal science 98:skaa092. 
  26. Mahboub HDH, Muller J, von Borell E. 2004. Outdoor use, tonic immobility, heterophil/lymphocyte ratio and feather condition in free-range laying hens of different genotype. British Poultry Science 45:738-744.  https://doi.org/10.1080/00071660400014267
  27. Mazzoni M, Zampiga M, Clavenzani P, Lattanzio G, Tagliavia C, Sirri F. 2022. Effect of chronic heat stress on gastrointestinal histology and expression of feed intake-regulatory hormones in broiler chickens. Animal 16:100600. 
  28. Meyer MM, Bobeck EA. 2023. Dietary vasodilator and vitamin C/L-arginine/choline blend improve broiler feed efficiency during finishing and reduce woody breast severity at 6 and 7 wks. Poultry Science 102:102421. 
  29. Mustafa A, Bai S, Zeng Q, Ding X, Wang J, Xuan Y, Su Z, Zhang K. 2021. Effect of organic acids on growth performance, intestinal morphology, and immunity of broiler chickens with and without coccidial challenge. AMB Express 11:140. 
  30. Nawab A, Ibtisham F, Li G, Kieser B, Wu J, Liu W, Zhao Y, Nawab Y, Li K, Xiao M, et al. 2018. Heat stress in poultry production: Mitigation strategies to overcome the future challenges facing the global poultry industry. Journal of Thermal Biology 78:131-139.  https://doi.org/10.1016/j.jtherbio.2018.08.010
  31. Nawaz AH, Amoah K, Leng QY, Zheng JH, Zhang WL, Zhang L. 2021. Poultry response to heat stress: Its physiological, metabolic, and genetic implications on meat production and quality including strategies to improve broiler production in a warming world. Frontiers in Veterinary Science 8:699081. 
  32. Oshima S, Shiiya S, Nakamura Y. 2019. Combined supplementation with glycine and tryptophan reduces purine-induced serum uric acid elevation by accelerating urinary uric acid excretion: A randomized, single-blind, placebo-controlled, crossover study. Nutrients 11:2562. 
  33. Ospina-Rojas IC, Murakami AE, Duarte CRA, Eyng C, Oliveira CAL, Janeiro V. 2014. Valine, isoleucine, arginine and glycine supplementation of low-protein diets for broiler chickens during the starter and grower phases. British Poultry Science 55:766-773.  https://doi.org/10.1080/00071668.2014.970125
  34. Oxford JH, Selvaraj RK. 2019. Effects of glutamine supplementation on broiler performance and intestinal immune parameters during an experimental coccidiosis infection. Journal of Applied Poultry Research 28:1279-1287.  https://doi.org/10.3382/japr/pfz095
  35. Park JS, Kang DR, Shim KS. 2022. Proteomic changes in broiler liver by body weight differences under chronic heat stress. Poultry Science 101:101794. 
  36. Razak MA, Begum PS, Viswanath B, Rajagopal S. 2017. Multifarious beneficial effect of nonessential amino acid, glycine: A review. Oxidative Medicine and Cellular Longevity 2017:1716701. 
  37. Renaudeau D, Collin A, Yahav S, de Basilio V, Gourdine JL, Collier RJ. 2012. Adaptation to hot climate and strategies to alleviate heat stress in livestock production. Animal 6:707-728.  https://doi.org/10.1017/S1751731111002448
  38. Saeed M, Yatao X, Hassan FU, Arain MA, Abd El-Hack ME, Noreldin AE, Sun C. 2018. Influence of graded levels of l-theanine dietary supplementation on growth performance, carcass traits, meat quality, organs histomorphometry, blood chemistry and immune response of broiler chickens. International Journal of Molecular Sciences 19:462. 
  39. Santos RR, Awati A, Roubos-van den Hil PJ, Tersteeg-Zijderveld MHG, Koolmees PA, Fink-Gremmels J. 2015. Quantitative histo-morphometric analysis of heat-stress-related damage in the small intestines of broiler chickens. Avian Pathology 44:19-22.  https://doi.org/10.1080/03079457.2014.988122
  40. Shafiekhani M, Ommati MM, Azarpira N, Heidari R, Salarian AA. 2019. Glycine supplementation mitigates lead-induced renal injury in mice. Journal of Experimental Pharmacology 11:15-22.  https://doi.org/10.2147/JEP.S190846
  41. Siegert W, Ahmadi H, Helmbrecht A, Rodehutscord M. 2015. A quantitative study of the interactive effects of glycine and serine with threonine and choline on growth performance in broilers. Poultry Science 94:1557-1568.  https://doi.org/10.3382/ps/pev109
  42. Sifa D, Bai X, Zhang D, Hu H, Wu X, Wen A, He S, Zhao L. 2018. Dietary glutamine improves meat quality, skeletal muscle antioxidant capacity and glutamine metabolism in broilers under acute heat stress. Journal of Applied Animal Research 46:1412-1417.  https://doi.org/10.1080/09712119.2018.1520113
  43. Son J, Lee WD, Kim HJ, Kang BS, Kang HK. 2022. Effect of providing environmental enrichment into aviary house on the welfare of laying hens. Animals 12:1165. 
  44. Swiatkiewicz S, Arczewska-Wlosek A, Jozefiak D. 2017. The nutrition of poultry as a factor affecting litter quality and foot pad dermatitis-an updated review. Journal of Animal Physiology and Animal Nutrition 101:e14-e20.  https://doi.org/10.1111/jpn.12630
  45. Valizadeh MR, Sadeghi AA, Chamani M, Shawrang P, Feizi F. 2014. The Effect of increasing dietary threonine to lysine ratio on carcass characteristics, mucin gene expression and morphological analysis of ileum of male broiler chickens challenged with Salmonella. International Journal of Biosciences 5:138-146. 
  46. Wang Z, Shao D, Kang K, Wu S, Zhong G, Song Z, Shi S. 2022. Low protein with high amino acid diets improves the growth performance of yellow feather broilers by improving intestinal health under cyclic heat stress. Journal of Thermal Biology 105:103219. 
  47. Wang Z, Zhao D, Chen L, Li J, Yuan G, Yang G, Zhang H, Guo X, Zhang J. 2019. Glycine increases glyoxalase-1 function by promoting nuclear factor erythroid 2-related factor 2 translocation into the nucleus of kidney cells of streptozotocin-induced diabetic rats. Journal of Diabetes Investigation 10:1189-1198.  https://doi.org/10.1111/jdi.13032
  48. Won SY, Han GP, Kwon CH, Lee EC, Kil DY. 2023. Effect of individual or combination of dietary betaine and glycine on productive performance, stress response, liver health, and intestinal barrier function in broiler chickens raised under heat stress conditions. Poultry Science 102:102771. 
  49. Xu X, Wang X, Wu H, Zhu H, Liu C, Hou Y, Dai B, Liu X, Liu Y. 2018. Glycine relieves intestinal injury by maintaining mTOR signaling and suppressing AMPK, TLR4, and NOD signaling in weaned piglets after lipopolysaccharide challenge. International Journal of Molecular Sciences 19:1980. 
  50. Xue GD, Barekatain R, Wu SB, Choct M, Swick RA. 2018. Dietary L-glutamine supplementation improves growth performance, gut morphology, and serum biochemical indices of broiler chickens during necrotic enteritis challenge. Poultry Science 97:1334-1341.  https://doi.org/10.3382/ps/pex444
  51. Yu M, Oketch EO, Hong JS, Nawarathne SR, Vohobjonov Y, Heo JM. 2022. Evaluation of preslaughter losses, meat quality, and physiological characteristics of broilers in response to crating density for the standard of animal welfare and to seasonal differences. Korean Journal of Agricultural Science 49:927-936.  https://doi.org/10.7744/KJOAS.20220084
  52. Yuan J, Karimi A, Zornes S, Goodgame S, Mussini F, Lu C, Waldroup PW. 2012. Evaluation of the role of glycine in low-protein amino acid-supplemented diets. Journal of Applied Poultry Research 21:726-737.  https://doi.org/10.3382/japr.2011-00388
  53. Zhang B, Lv Z, Li Z, Wang W, Li G, Guo Y. 2018. Dietary L-arginine supplementation alleviates the intestinal injury and modulates the gut microbiota in broiler chickens challenged by Clostridium perfringens. Frontiers in Microbiology 9:1716. 
  54. Zhang C, Wang C, Zhao X, Chen K, Geng Z. 2020. Effect of L-theanine on meat quality, muscle amino acid profiles, and antioxidant status of broilers. Animal Science Journal 91:e13351.