The Journal of Internal Korean Medicine (대한한방내과학회지)

The Society of Internal Korean Medicine (대한한방내과학회)
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Study of the Effect and Underlying Mechanism of Enzyme-Treated Garlic Extract on a Lipopolysaccharide-Induced Acute Colitis Model

효소처리를 이용한 마늘 추출물이 Lipopolysaccharide 유발 급성 장염 모델에 미치는 효과 및 기전 연구

  • Min Ju Kim (Research Center for Herbal Convergence on Liver Disease, Daegu Haany University) ;
  • Mi-Rae Shin (Dept. of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Hak Joo Choi (Dept. of Herbology, College of Korean Medicine, Daegu Haany University) ;
  • Seong-Soo Roh (Dept. of Herbology, College of Korean Medicine, Daegu Haany University)
  • 김민주 (대구한의대학교 간질환한약융복합활용연구센터) ;
  • 신미래 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 최학주 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 노성수 (대구한의대학교 한의과대학 본초약리학교실)
  • Received : 2023.11.30
  • Accepted : 2024.01.03
  • Published : 2023.12.30
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Abstract

Objective: This study aims to explore the pharmacological effects and mechanisms of enzyme (Viscozyme)-treated garlic extract (EG) in an animal model of acute enteritis induced by lipopolysaccharide (LPS). Methods: The experiment included four subgroups: normal, control, EG200 (treated with 200 mg/kg EG), and EG400 (treated with 400 mg/kg EG). Drug administration lasted 3 days, followed by the induction of acute enteritis in all groups (except normal) through the intraperitoneal administration of 20 mg/kg of LPS 1 h after the last oral dose. Autopsy was conducted 24 h later to collect serum and colon tissue. Serum was analyzed for reactive oxygen species (ROS) and C-reactive protein (CRP), while Western blotting was performed on the colon tissue. Results: After analyzing the ROS and CRP levels in serum, the EG treatment group exhibited a significant decrease compared with the control group. The EG treatment group exhibited a significant decrease in the activation of the mitogen-activated protein kinases (MAPKs)/nuclear factor-kappa B p65 (NF-κB) pathway compared with the control group. EG administration significantly regulated apoptosis-related factors, including B-cell lymphoma-2 (Bcl-2), Bcl-2-associated X, cysteine aspartyl-specific protease-3, and cytochrome C. Conclusions: EG treatment in mice with LPS-induced acute colitis reduced the ROS and CRP levels, suppressed the MAPKs/NF-κB pathway in the colon, and effectively alleviated acute enteritis by modulating apoptosis-related factors. Based on these findings, EG emerges as a promising candidate for the prevention and treatment of acute colitis, showing its potential therapeutic efficacy in this experimental model.

Keywords

Acknowledgement

본 연구는 중소벤처기업부의 기술개발사업[S3281126]과 정부 (과학기술정보통신부)의 재원으로 한국연구재단의 지원을 받아 수행되었습니다(No. 2018R1A5A2025272).

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