Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Effects of lactic acid bacteria fermented feed and three types of lactic acid bacteria (L. plantarum, L. acidophilus, B. animalis) on intestinal microbiota and T cell polarization (Th1, Th2, Th17, Treg) in the intestinal lymph nodes and spleens of rats

  • Da Yoon, Yu (Division of Animal Science, Gyeongsang National University) ;
  • Sang-Hyon, Oh (Division of Animal Science, Gyeongsang National University) ;
  • In Sung, Kim (Division of Animal Science, Gyeongsang National University) ;
  • Gwang Il, Kim (Division of Animal Science, Gyeongsang National University) ;
  • Jeong A, Kim (Division of Animal Science, Gyeongsang National University) ;
  • Yang Soo, Moon (Division of Animal Bioscience & Integrated Biotechnology, Gyeongsang National University) ;
  • Jae Cheol, Jang (Division of Animal Science, Gyeongsang National University) ;
  • Sang Suk, Lee (Department of Animal Science and Technology, Sunchon National University) ;
  • Jong Hyun, Jung (Jung P&C Institute) ;
  • Hwa Chun, Park (Dasan Genetics) ;
  • Kwang Keun, Cho (Division of Animal Science, Gyeongsang National University)
  • Received : 2022.08.05
  • Accepted : 2022.09.16
  • Published : 2023.01.01
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Abstract

Objective: In this study, we investigated the effects of Rubus coreanus-derived lactic acid bacteria (LAB) fermented feed (RC-LAB fermented feed) and three types of LAB (Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium animalis) on the expression of transcription factors and cytokines in Th1, Th2, Th17, and Treg cells in the intestinal lymph nodes and spleens of rats. In addition, the effect on intestinal microbiota composition and body weight was investigated. Methods: Five-week-old male rats were assigned to five treatments and eight replicates. The expression of transcription factors and cytokines of Th1, Th2, Th17, and Treg cells in the intestinal lymph nodes and spleens was analyzed using real-time reverse transcriptase polymerase chain reaction assays. Intestinal tract microbiota compositions were analyzed by next-generation sequencing and quantitative polymerase chain reaction assays. Results: RC-LAB fermented feed and three types of LAB increased the expression of transcription factors and cytokines in Th1, Treg cells and Galectin-9, but decreased in Th2 and Th17 cells. In addition, the intestinal microbiota composition changed, the body weight and Firmicutes to Bacteroidetes (F/B) ratio decreased, and the relative abundance of LAB increased. Conclusion: LAB fermented feed and three types of LAB showed an immune modulation effect by inducing T cell polarization and increased LAB in the intestinal microbiota.

Keywords

Acknowledgement

This research was supported by the Economic Cooperation Industrial Development Project (No. R0005730), Ministry of Trade, Industry, and Energy, Korea.

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