한국동물위생학회지 (Korean Journal of Veterinary Service)

  • 제46권4호
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  • Pages.303-314
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  • 2023
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  • 3022-7372(pISSN)
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  • 2287-7630(eISSN)
한국동물위생학회 (The Korean Society of Veterinary Service)
권호 표지
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산란계에서 Chlorhexidine-inactivated Salmonella Enteritidis, S. Typhimurium 및 S. Gallinarum 3가 백신의 효능평가

Evaluation of the protective efficacy of trivalent Salmonella inactivated vaccine including Chlorhexidine-inactivated Salmonella Enteritidis, S. Typhimurium and S. Gallinarum in poultry

  • 유영주 (전북대학교 수의과대학 수의공중보건학실) ;
  • 유정희 (전북대학교 수의과대학 수의공중보건학실) ;
  • 허진 (전북대학교 수의과대학 수의공중보건학실)
  • Yeong Ju Yu (Department of Veterinary Public Health, College of Veterinary Medicinee, Jeonbuk National University) ;
  • Jeong Hee Yu (Department of Veterinary Public Health, College of Veterinary Medicinee, Jeonbuk National University) ;
  • Jin Hur (Department of Veterinary Public Health, College of Veterinary Medicinee, Jeonbuk National University)
  • 투고 : 2023.11.28
  • 심사 : 2023.12.07
  • 발행 : 2023.12.30
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초록

Protective efficacy of trivalent Salmonella inactivated vaccine containing Chlorhexidine-inactivated S. Enterltidis (SE), S. Typhimurium (ST), and S. Gallinarum (SG) strains, was evaluated in this study. A total of 70 brown nick layers were divided into 7 groups, A to G, containing 10 hens per group. All hens in groups B to D were intramuscularly immunized with approximately 7×108 cells (3×108 cells of SE+1×108 SE+1×108 cells of ST+3×108 cells of SG) of the trivalent vaccine in 0.5 mL of PBS. All chickens in groups E to G were injected with sterile PBS. All hens of groups B and E, groups C and F, and groups D and G were orally challenged with approximately 2 ×109 CFU of wild-type SE, ST, and SG, respectively. Serum IgG titers and CD3+CD4+ T-cells, and CD3+CD8+ T-cells levels of groups B to D significantly higher than those of group A. In addition, all animals in groups A to C, E and F showed no clinical symptoms and survived after the virulent challenges, whereas one chicken in group D died and all chickens in group G died following the challenge. The protection against wild-type SE and ST in liver, spleen, cecum, and cloaca of groups B and C chickens was significant effective as compared with those in groups E and F. These indicate that the trivalent inactivated vaccine can be an effective tool for prevention of Salmonella infections by inducing robustly protective immune responses and cellular immune response in chickens.

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과제정보

이 논문은 정부의 재원으로 농림식품기술기획평가원(Korea Institute of Planning and Evaluation for Technology in Food, Agriculture and Forestry, IPET)의 지원을 받아 연구되었습니다(No. 122007-2).

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